TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples
Published Online: Jun 16, 2023
Page range: 187 - 195
Received: Oct 27, 2022
Accepted: Apr 18, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0024
Keywords
© 2023 Mohammad Sabri Abdul Rahman et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated with kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation and determine the severity of infection, especially in the acute stage, allowing early detection for immediate treatment decisions. To our knowledge, real-time PCR has not been routinely adopted for clinical investigation in Malaysia. This study evaluated TaqMan real-time PCR (qPCR) assays diagnosing leptospirosis and compared their applicability to clinical samples from dogs with kidney and/or liver disease against a conventional PCR reference.
Material and Methods
The qPCR assays were validated using existing leptospiral isolates. Whole blood and urine samples were analysed using a conventional PCR,
Results
The
Conclusion
The