Antibody response to enterotoxigenic Bacteroides fragilis of Filipino colorectal cancer patients
Article Category: Brief communication
Published Online: Dec 28, 2023
Page range: 273 - 280
DOI: https://doi.org/10.2478/abm-2023-0070
Keywords
© 2023 Ana Maria D. Cariño et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Several species of the gut microbiota have been implicated in colorectal cancer (CRC) development. The anaerobic bacterium enterotoxigenic
Objective
To determine the antibody response of CRC patients against ETBF to ascertain whether significant difference exists or whether antibody response is related to tumor grade and tumor stage.
Methods
Informed consent was obtained from histologically confirmed CRC casesand their age- and sex-matched clinically healthy controls. Plasma samples from the participants were subjected to in-house enzyme-linked immunosorbent assay (ELISA) to determine their antibody levels.
Results
Using ETBF total protein as coating antigen, 38/39 (97%) CRC cases and 36/39 (92%) controls showed anti-ETBF IgG above cut-off, while all (100%) CRC cases and 36/39 (92%) controls had anti-ETBF IgA levels above cut-off. With culture broth as coating antigen, all (100%) CRC cases and 37/39 (95%) controls had anti-ETBF IgG levels above cut-off. For anti-ETBF IgA, all (100%) cases and controls had levels above cut-off. Statistical analysis reveals no significant difference (
Conclusions
These results suggest that Filipino CRC cases and their clinically healthy matched controls exhibit antibody responses against ETBF.