Abstract
Highly sensitive and simple spectrophotometric determination of selenium is described for the determination of selenium(IV) using a new reagent leuco malachite green. The method is based on the reaction of selenium(IV) with potassium iodide in an acidic condition to liberate iodine, the liberated iodine oxidizes leuco malachite green to malachite green dye. The green coloration was developed in an acetate buffer (pH 4.2–4.9) on heating in a water bath (∼ 40 °C). The formed dye exhibits an absorption maximum at 615 nm. The method obeys Beer’s law over a concentration range of 0.04–0.4 µg mL−1 selenium. The molar absorptivity and Sandell’s sensitivity of the color system were found to be 1.67 × 105 L mol−1 cm-1 and 0.5 ng cm−2, respectively. The optimum reaction conditions and other analytical parameters have been evaluated. The proposed procedure has been successfully applied to the determination of selenium in real samples of water, soil, plant material, human hair, and cosmetic samples. The results were compared to those obtained with the reference method. Statistical analysis of the results confirms the precision and accuracy of the proposed method. In addition, the developed method is cost-effective and involves easily accessible instrumentation technique which can be used by ordinary research laboratories.
[1] X. Ziegel and A. Ziegel (Eds.): Concepts for Metal Ion Toxicity, Dekker, New York, 1986. Search in Google Scholar
[2] H. Parkman and H. Hultberg: “Occurrence and effects of selenium in the environment — a literature review”, IVL-Rapport (Swedish), Vol. B1486, (2002), pp. 1–26. Search in Google Scholar
[3] J.E. Spallholz, J.L. Martin and H.E. Ganther (Eds.): Selenium in Biology and Medicine, AVI Publishing Co. Inc., Westport, CT, 1981. Search in Google Scholar
[4] D. Wang, G. Alfthan, A. Aro, P. Lahermo and P. Vaananen: “The impact of selenium fertilization on the distribution of selenium in rivers in Finland”, Agri. Ecosyst. Environ., Vol. 50, (1994), pp. 133–149. http://dx.doi.org/10.1016/0167-8809(94)90132-510.1016/0167-8809(94)90132-5Search in Google Scholar
[5] F.A. Patty: Industrial Hygiene and Toxicology, Vol. II, Wiley Interscience, New York, 1962. Search in Google Scholar
[6] A.D. Shendrikar and P.W. West: “Air sampling methods for the determination of selenium”, Anal. Chim. Acta, Vol. 89, (1977), pp. 403–406. http://dx.doi.org/10.1016/S0003-2670(01)84740-110.1016/S0003-2670(01)84740-1Search in Google Scholar
[7] J.R. Shapira: Organic Selenium Compounds their Chemistry and Biology, Wiley Interscience, New York, 1971. Search in Google Scholar
[8] APHA: Standard Methods for the Examination of Water and Wastewater, 19th ed., American Public Health Association, Washington, D.C., 1995. Search in Google Scholar
[9] L.K. Isaeev (Ed.): Control of Chemical and Biological Environmental Parameters, St. Petersburg, Krismas, 1998. Search in Google Scholar
[10] G.P. Bespamyatnov and Yu.A. Krotov: Maximum Permissible Concentrations of Chemical Compounds in the Environment, Khimiya, Moscow, 1985. Search in Google Scholar
[11] V. Badmaev and M. Majeed: “Selenium — a quest for better understanding”, Altern. Therap., Vol. 2, (1996), pp. 59–67. http://dx.doi.org/10.1089/act.1996.2.5910.1089/act.1996.2.59Search in Google Scholar
[12] M. Navarrete, A. Gaudry, G. Revel, T. Martinez and L. Cabrera: “Urinary selenium excretion in patients with cervical uterine cancer”, Biol. Trace Elem. Res., Vol. 79, (2001), pp. 97–105. http://dx.doi.org/10.1385/BTER:79:2:9710.1385/BTER:79:2:97Search in Google Scholar
[13] E. Meuillet, S. Stratton, D.P. Cherkuri, A.C. Goullet, J. Kagey, B. Porterfield and M.A. Nelson: “Chemoprevention of prostate cancer with selenium — an update on current clinical trials and preclinical findings”, J. Cell. Biochem., Vol. 91, (2004), pp. 443–458. http://dx.doi.org/10.1002/jcb.1072810.1002/jcb.10728Search in Google Scholar
[14] L.K. Schumacher and M. Roy: “Effects of selenium on the immunocompetence of patients with head and neck cancer on adoptive immunotherapy of early and established lesions”, BioFactors, Vol. 14, (2001), pp. 161–168. Search in Google Scholar
[15] G.F. CombsJr., L.C. Clark and B.W. Turnbull: “An analysis of cancer prevention by selenium”, Biofactors, Vol. 14, (2001), pp. 153–159. Search in Google Scholar
[16] L.R. McDowell: Minerals in Animals and Human Nutrition, Academic Press, New York, 1992. Search in Google Scholar
[17] J. Tan and Y. Huang: “Selenium in geo-ecosystem and its relation to endemic diseases in China”, Water Air & Soil Pollut., Vol. 57–58, (1991), pp. 59–68. http://dx.doi.org/10.1007/BF0028286910.1007/BF00282869Search in Google Scholar
[18] R.L. Tatken and R.J. Lewis: Registry of Toxic Effects of Chemical Substances, US Dept. Health and Human Science, Cincinnati, Ohio, 1983. Search in Google Scholar
[19] K. Yang and L. Husain: “Ultratrace determination of selenium by HG-ICP-MS operated under nanobust plasma conditions”, Spec. Lett., Vol. 39, (2006), pp. 187–201. http://dx.doi.org/10.1080/0038701050053123310.1080/00387010500531233Search in Google Scholar
[20] M. Montes Bayon, M. Diaz Molet, E.B. Gonzalez and A. Sanz-Medel: “Evaluation of different sample extraction strategies for selenium determination in seleniumenriched plants (Allium sativum and Brassica juncea) and Se speciation by HPLC-ICP-MS”, Talanta, Vol. 68, (2006), pp. 1287–1293. http://dx.doi.org/10.1016/j.talanta.2005.07.04010.1016/j.talanta.2005.07.040Search in Google Scholar
[21] D. Majez, I. Falnoga, M. Veber and V. Stibilj: “Determination of selenium species in plant leaves by HPLC-UV-HG-AFS”, Talanta, Vol. 68, (2006), 1287–1293. http://dx.doi.org/10.1016/j.talanta.2005.07.04010.1016/j.talanta.2005.07.040Search in Google Scholar
[22] M. Murillo, N. Carrion, M. Quintana, G. Sanabria, M. Rios, L. Duarte and F. Ablan: “Determination of selenium and iodine in human thyroids”, J. Trace Elem. Med. and Biol., Vol. 19, (2005), pp. 23–27. http://dx.doi.org/10.1016/j.jtemb.2005.07.00510.1016/j.jtemb.2005.07.005Search in Google Scholar
[23] U.M. El-Ghawi, A.A. Al-Sadeq, M.M. Bejey and M.B. Alamin: “Determination of selenium in Libyan food items using pseudocyclic instrumental neutron activation analysis”, Biol. Trace Elem. Res., Vol. 107, (2005), pp. 61–171. http://dx.doi.org/10.1385/BTER:107:1:06110.1385/BTER:107:1:061Search in Google Scholar
[24] S. Theodorolea, N.S. Thomaidis and E. Piperaki: “Determination of selenium in human milk by electrothermal atomic absorption spectrometry and chemical modification”, Anal. Chim. Acta, Vol. 547, (2005), pp. 132–137. http://dx.doi.org/10.1016/j.aca.2005.01.03010.1016/j.aca.2005.01.030Search in Google Scholar
[25] N.Yu. Stozhko, E.I. Morosanova, L.I. Kolyadina and S.V. Fomina: “Ceramic composite electrode for the determination of selenium(IV) by stripping voltammetry”, J. Anal. Chem., Vol. 61, (2006), pp. 158–165. http://dx.doi.org/10.1134/S106193480602012210.1134/S1061934806020122Search in Google Scholar
[26] S. Nakano, M. Yoshii and T. Kawashima: “Flow-injection simultaneous determination of selenium(IV) and selenium(IV + VI) using photooxidative coupling of p-hydrazinobensenesulfonic acid with N-(1-naphthyl)ethylene diamine”, Talanta, Vol. 64, (2004), pp. 1266–1272. http://dx.doi.org/10.1016/j.talanta.2004.04.03310.1016/j.talanta.2004.04.033Search in Google Scholar
[27] Z. Marczenko: Separation and Spectrophotometric Determination of Elements, 2nd ed., Ellis Horwood, Chichester, England, 1986. Search in Google Scholar
[28] I. Nemcova, L. Cermakova and J. Gasparic: Spectrophotometric Reactions, Marcell Dekker Inc., New York, 1996. Search in Google Scholar
[29] I.S. Balogh and V. Andruch: “1,3,3-Trimethyl-2-[3-(1,3,3-trimethyl-1,3-dihydroindol-2-ylidene)propenyl]-3H-indolium chloride, a highly sensitive reagent for the spectrophotometric determination of selenium”, Chem. Papers, Vol. 59, (2005), pp. 347–349. Search in Google Scholar
[30] B. Narayan, M. Mathew, N.G. Bhat and N.V. Sreekumar: “Spectrophotometric Determination of Selenium Using Potassium Iodide and Starch as Reagents”, Microchim. Acta, Vol. 141, (2003), pp. 175–178. http://dx.doi.org/10.1007/s00604-002-0936-210.1007/s00604-002-0936-2Search in Google Scholar
[31] H.D. Revanasiddappa and T.N. Kiran Kumar: “Spectrophotometric determination of selenium by use of thionin”, Anal. Bioanal. Chem., Vol. 374, (2002), pp. 1121–1124. http://dx.doi.org/10.1007/s00216-002-1581-710.1007/s00216-002-1581-7Search in Google Scholar
[32] A. Afkhami and T. Madrakian: “Kinetic-spectrophotometric determination of selenium in natural water after preconcentration of elemental selenium on activated carbon”, Talanta, Vol. 58, (2002), pp. 311–317. http://dx.doi.org/10.1016/S0039-9140(02)00246-110.1016/S0039-9140(02)00246-1Search in Google Scholar
[33] U. Ornenark and A. Olin: “Determination of dissolved selenium(VI) in freshwater”, Talanta, Vol. 41, (1994), pp. 1675–1681. http://dx.doi.org/10.1016/0039-9140(94)E0099-D10.1016/0039-9140(94)E0099-DSearch in Google Scholar
[34] K.N. Ramachandran, R. Kaveeshwar and V.K. Gupta: “Spectrophotometric determination of selenium with 6-amino-1-naphthol-3-sulphonic acid (J-acid) and its application in trace analysis”, Talanta, Vol. 40, (1993), pp. 781–784. http://dx.doi.org/10.1016/0039-9140(93)80030-U10.1016/0039-9140(93)80030-USearch in Google Scholar
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