They say that the increase of pocket fluid combine with aggravation of periodontal disease. This mechanism and component are generally considered, however, the lipid substance in exudate still remains unknown, except for the report of WATANABE. His report contained that the amount of triglyceride in human serum and pocket fluid tend to increase, on the other hand in human gingiva was tend to decrease in propotion to the aggravation of the disease.
From his report, the authers investigate about the amount of diglyceride (DG) and monoglyceride (MG).
By way of study material, a total of 29 patients (13 males and 16 females) who visited the Department of Periodontology, Nihon University Dental Hospital, were selectively used. The subjects, who ranged from 20 to 72 years in age, were free from any systemic disease except for some kind of periodontal complaint.
The areas of pocket fluid collection (facial of 3-3 or 3-3) studied according to PMA index. In collecting the exudate from the periodontal pockets, filter paper strips (Whatman Co., No. 2, 1×12mm) was used. Total lipids were extracted with chloroform/methanol (2: 1v/v). The separation of the extract was achieved by thin-layer chromatography. The amount of diglyceride and monoglyceride in them was determined by the enzyme method-the endpoint method (reagents: Boehringer Mannheim) using the ultraviolet absorption spectrophotometry.
As a result of the study, the authors arrived at the following conclusions:
1. In this study identified that DG and MG were concluded in pocket fluid by use of thin-layer chromatography.
2. The amount of DG and MG were inclined to increase in proportion to the aggravation of the disease.