Fatigue test was carried out on Ni-based bulk metallic glass (BMG) for which there is still no reports on fatigue strength. Test alloy rods with a diameter of 2 mm were prepared in Ni₆₀Zr₂₀Nb₁₅Al₅ at% systems by copper mold casting method. The tensile strength (σ_B) was 2.9 GPa. The test specimens were machined to hourglass shape type with a minimum diameter of 0.9 mm. The specimens were tested at a stress ratio of 0.1 and a frequency of 10 Hz. The fatigue limit (σ_W) and fatigue ratio (σ_W/σ_B) in the Ni-based BMG showed high values of 1.68 GPa and 0.58, respectively. The tendency that the σ_W increased with increasing the σ_B in BMGs was indicated in the Ni-based BMG. In addition, the fatigue fracture toughness in the Ni-based BMG showed a high value of 14 MPa•m^1/2.