Srpski arhiv za celokupno lekarstvo 2006 Volume 134, Issue Suppl. 1, Pages: 9-16
https://doi.org/10.2298/SARH06S1009L
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Cytohistological and immunohistochemical characteristics of vascular remodelling in diseases of the blood vessels
Lačković Vesna (School of Medicine, Institute of Histology and Embryology, Belgrade)
Vuković Irena (School of Medicine, Institute of Histology and Embryology, Kragujevac)
INTRODUCTION. Vascular remodelling is an adaptive process involving the
adjustment of the structure and function of blood vessels to long-term
changes in haemodynamic conditions. This process leads to structural
alterations within vessel walls in different cardiovascular diseases, such as
hypertension, atherosclerosis, and coarctation of the aorta. OBJECTIVE. We
investigated the histochemical and immunocytochemical characteristics of
morphological lesions in coronary atherosclerosis and coarctation of the
aorta. METHOD. Twenty-one samples of atherosclerotically modified right
coronary arteries, divided into 6 segments, were analysed. We also examined
10 samples of coarctation segments, excised during surgery. The segments were
stained histochemically (using orcein and alcian blue-PAS),
immunocytochemically (using α-smooth muscle actin-α-SMA, vimentin, desmin,
myosin heavy chains-MHC, CD3, CD45, S-100, and Proliferating Cell Nuclear
Antigen-PCNA), and for electron microscopy. RESULTS. The results of our study
of morphological lesions in coronary atherosclerosis demonstrated initial
functional and then, in the later stages of atherosclerosis, morphological,
damage to the endothelium. The preatheroma stage revealed the presence of
intimal dedifferentiation of smooth muscle cells, with the expression of
vimentin and α-SMA, and the lack of expression of desmin. Along with these
changes, a huge number of foam cells of variant origin were noticed. Some of
them were CD68-immunoreactive while others were both vimentin- and
S-100-immunoreactive. All examined samples of the coarctation of the aorta
demonstrated the presence of dedifferentiated smooth muscle cells as well as
a diminution in cell numbers, followed by apoptotic smooth muscle cells, and
the absence of inflammatory cells. CONCLUSION. Some foam cells develop from
monocytemacrophage lineage (CD68-immunoreactive), while others originate from
smooth muscle cells (vimentin and S-100- immunoreactive). Coarctation of the
aorta is characterised by a diminution in cell numbers (apoptosis) as well as
their dedifferentiation from contractile to synthetic phenotype.
Keywords: atherosclerosis, coarctation, smooth muscle cells, intimal thickening