doiSerbia

Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells

Radojičić Ratko M. (Institute of Physiology and Biochemistry, Faculty of Biology, Belgrade)
Spasić Snežana D. (Center for Chemistry ICTM, Belgrade)
Saičić Zorica S. (Institute for Biological Research 'Siniša Stanković', Department of Physiology, Belgrade)
Jovanović Tamara B. (Fullerene Science and Technology Centre, Chemical Power Sources Institute, Belgrade-Zemun, Serbia and Montenegro)
Simić-Krstić Jovana B. (Fullerene Science and Technology Centre, Chemical Power Sources Institute, Belgrade-Zemun, Serbia and Montenegro)

The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.

Keywords: B-16 mouse melanoma, isoenzymes, α-naphthyl acetate esterase (ANAE), leucine aminopeptidase (LAP), copper, zinc–containing superoxide dismutase (CuZn SOD).