Acta Periodica Technologica 2016 Issue 47, Pages: 249-263
https://doi.org/10.2298/APT1647249Z
Full text ( 884 KB)
Cited by
Optimization of ultrasound-assisted extraction of polyphenolic compounds from coriander seeds using response surface methodology
Zeković Zoran P. (Faculty of Technology, Novi Sad)
Đurović Saša D. (Faculty of Technology, Novi Sad)
Pavlić Branimir M. (Faculty of Technology, Novi Sad)
Coriandrum sativum L. (coriander) seeds (CS) were used for preparation of
extracts with high content of biologically active compounds. In order to
optimize ultrasoundassisted extraction process, three levels and three
variables of Box-Behnken experimental design (BBD) in combination with
response surface methodology (RSM) were applied, yielding maximized total
phenolics (TP) and flavonoids (TF) content and antioxidant activity (IC50 and
EC50 values). Independent variables were temperature (40-80oC), extraction
time (40-80 min) and ultrasonic power (96-216 W). Experimental results were
fitted to a second-order polynomial model with multiple regression, while the
analysis of variance (ANOVA) was employed to assess the model fitness and
determine optimal conditions for TP (79.60oC, 49.20 min, 96.69 W), TF
(79.40oC, 43.60 min, 216.00 W), IC50 (80.00oC, 60.40 min, 216.00 W) and EC50
(78.40oC, 68.60 min, 214.80 W). On the basis of the obtained mathematical
models, three-dimensional surface plots were generated. The predicted values
for TP, TF, IC50 and EC50 were: 382.68 mg GAE/100 g CS, 216 mg CE/100 g CS,
0.03764 mg/mL and 0.1425 mg/mL, respectively.
Keywords: coriander seeds, ultrasound-assisted extraction, optimization, response surface methodology
Projekat Ministarstva nauke
Republike Srbije, br. TR31013