About the journal

Cobiss

Srpski arhiv za celokupno lekarstvo 2022 Volume 150, Issue 7-8, Pages: 400-406
https://doi.org/10.2298/SARH220402070S
Full text ( 1092 KB)


Different angiogenic response and bone regeneration following the use of various types of collagen membranes - in vivo histomorphometric study in rabbit calvarial critical-size defects

Stepić-Hajdarpašić Jelena (University of Belgrade, School of Dental Medicine, Clinic for Oral Surgery, Belgrade, Serbia), stepic.jelena@gmail.com
Brković Božidar (University of Belgrade, School of Dental Medicine, Clinic for Oral Surgery, Belgrade, Serbia)
Dragović Miroslav ORCID iD icon (University of Belgrade, School of Dental Medicine, Department of Medical Statistics and Informatics, Belgrade, Serbia)
Pejović Marko (University Business Academy in Novi Sad, Faculty of Dentistry, Clinic for Oral Surgery and Implantology, Pančevo, Serbia)
Sopta Jelena (University of Belgrade, Faculty of Medicine, Department of Pathology, Belgrade, Serbia)
Kuzmanović-Pfićer Jovana ORCID iD icon (University of Belgrade, School of Dental Medicine, Department of Medical Statistics and Informatics, Belgrade, Serbia)
Čolić Snježana (University of Belgrade, School of Dental Medicine, Clinic for Oral Surgery, Belgrade, Serbia)

Introduction/Objective. Success of guided bone regeneration depends on the size and morphology of defect, characteristics of barrier membranes and adequate angiogenesis. The aim of the study was to reveal impact of three different collagen membranes on angiogenesis and bone production in critical-size defects. Methods. Defects were created in rabbit calvarias, filled with bovine bone graft and randomly covered with one of three investigated collagen membranes (Biogide – BG, Heart – PC, Mucograft – MG) or left without a membrane for the control group (C). After two and four weeks of healing, a total of 10 animals were sacrificed for histological and histomorphometric analysis of angiogenesis, bone regeneration, and inflammatory response. Results. In the early healing phase, the highest values of trabecular thickness and trabecular area were recorded with PC and BG membranes, respectively. After four weeks, significantly improved bone healing was noted in the MG group, as well as significantly pronounced inflammation. Initially, vessel density was significantly higher in the C group compared to all three membranes. After four weeks, significantly better results were observed in the MG compared to the other groups, BG compared to the rest of groups, and between PC and C groups. Conclusion. The use of collagen membranes significantly affects angiogenesis, reducing it in the early and enhancing it at the later healing phase. All three tested membranes in combination with bone graft significantly improved the amount of regenerated bone. Among the investigated groups, MG favored more pronounced angiogenic, osteogenic, and inflammatory response in the observation period of four weeks.

Keywords: collagen membrane, angiogenesis, guided bone regeneration, collagen matrix, pericardium

Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175021

Show references