doiSerbia

Hypoglycemic effect of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)

Mikov Ivan (Institute of Occupational Health, Medical Faculty, Novi Sad)
Vasović Velibor (Department of Pharmacology, Toxicology and Clinical Pharmacology, Medical Faculty, Novi Sad)
Mikov Aleksandra (Institute of Child and Youth Health Care, Medical Faculty, Novi Sad)
Goločorbin-Kon Svetlana (Department of Biochemistry, Medical Faculty, Novi Sad)
Stankov Karmen (Department of Pharmacy, Medical Faculty, Novi Sad)
Mikov Momir (Department of Pharmacology, Toxicology and Clinical Pharmacology, Medical Faculty, Novi Sad)

Herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), a synthetic auxin which promotes uncontrolled plant growth is widely used. The aim of our study was to investigate, using an experimental model, the effect of herbicide 2,4-D on liver function tests, enzyme a amylase and glucose blood level. BALB/C mice were treated i.p. with the herbicide (30 mg/ kg 2,4-D) for four consecutive days. Twenty-four hours after the last injection, the treated and the control animals were weighed and sacrificed for biochemical analysis: haematocrit, glucose blood level, serum activities of enzymes alanine aminotransferase, aspartate aminotransferase, gamma glutamyl transferase, and a amylase, as well as liver reduced glutathione. Herbicide 2,4-D significantly decreased glucose blood level in mice. There were no changes in liver function tests or activity of enzyme a amylase. In this study on mice we confirmed the results obtained in the previous study, which showed a hypoglycemic effect of herbicide 2,4-D on agricultural workers. To elucidate the mechanism of this effect, a further research is needed.

Keywords: 2,4-Dichorophenoxyacetic acid, herbicide, glucose, liver, mice