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Journal of the Serbian Chemical Society 2021 Volume 86, Issue 12, Pages: 1205-1218
https://doi.org/10.2298/JSC210812086S
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Application of LC-MS/MS with ion mobility for chemical analysis of propolis extracts with antimicrobial potential

Sofrenić Ivana ORCID iD icon (University of Belgrade, Faculty of Chemistry, Belgrade, Serbia)
Ljujić Jovana (University of Belgrade, Faculty of Chemistry, Belgrade, Serbia)
Simić Katarina ORCID iD icon (University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia)
Ivanović Stefan ORCID iD icon (University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia)
Stanković-Jeremić Jovana ORCID iD icon (University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia)
Ćirić Ana ORCID iD icon (University of Belgrade, Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia)
Soković Marina ORCID iD icon (University of Belgrade, Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia)
Anđelković Boban ORCID iD icon (University of Belgrade, Faculty of Chemistry, Belgrade, Serbia), aboban@ch.bg.ac.rs

The objective of this study was to test four-dimensional LC-ESI-MS/ /MS chromatography in analysis of complex mixture such as ethanol extracts of different propolis samples. In total more than 1200 picks were identified and only for 185 literature conformation was found. The given data represent the result of tentative identification, and summarized results are given in the text. Comparing the samples, from different altitudes, 96 components were detected as characteristic in high altitude samples and 18 in samples collected at low altitudes. Antimicrobial activity of ethanol extracts of propolis (EEP) and propylene glycol extracts of propolis (PGEP) were carried out on S. aureus, B. cereus, M. flavus, L. monocytogenes, P. aeruginosa, S. typhimurium, E. coli and E. cloacae bacterial strains and compared with broad-spectrum antibiotics, streptomycin and ampicillin. Anti-quorum sensing activity was performed on P. aeruginosa by testing the effect of representative propolis extracts on biofilm formation, twitching and motility activity and production of pyocyanin. We demonstrated that the majority of explored propolis extracts have greater or equal minimal inhibitory concentration and minimum bactericidal concentration values compared to antibiotics, independently of the solvent used for the extraction. The samples collected from the highest altitude emerged as least active antimicrobial agents but with the greatest potential as anti-quorum sensing agents.

Keywords: tandem mass spectrometry, parallel accumulation serial fragmentation ion mobility mass spectrometer, poplar propolis, quorum sensing, P. aeruginosa

Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-9/2021-14/200168, Grant no. 451-03-9/2021-14/200026 and Grant no. 451-03-9/2021-14/200007