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Determination of methylparaben from cosmetic products by ultra performance liquid chromatography

Mincea Manuela M. (West University of Timisoara, Faculty of Chemistry-Biology-Geography, Department of Chemistry, Timisoara, Romania + Institute of Public Health Timisoara, Dept. of Food Hygiene, V. Babes, Timisoara, Romania)
Lupşa Ioana R. (Institute of Public Health Timisoara, Dept. of Food Hygiene, V. Babes, Timisoara, Romania)
Cinghiţă Dan F. (West University of Timisoara, Faculty of Chemistry-Biology-Geography, Department of Chemistry, Timisoara, Romania)
Radovan Ciprian V. (West University of Timisoara, Faculty of Chemistry-Biology-Geography, Department of Chemistry, Romania Timisoara, Romania)
Talpos Ioan (West University of Timisoara, Multidisciplinary Research Platform 'Nicholas Georgescu - Roegen', Timisoara, Romania)

A new method for the determination of methylparaben by ultra-performance liquid chromatography (UPLC) was developed. Methylparaben is often used as preservative, alone or in combination with other parabens, being added to cosmetic products, pharmaceutical products and foods to avoid microbial contamination. Due to its widespread use and potential risk to human health, assessing human exposure to this compound is of interest. A good determination and quantification of methylparaben was developed with a gradient elution using a mixture of methanol and water (60:40, v/v) within 1.455 min. Under optimized conditions, the linear working range extends over two orders of magnitude with relative standard deviations of intra- and inter-day precision below 2.3 %, and a detection limit of 0.02 ng μL-1 for methylparaben. The proposed method was successfully applied to the assay of methylparaben in cosmetic products with minimal sample preparation.

Keywords: UPLC, methylparaben, preservative, cosmetic products