Hemijska industrija 2014 Volume 68, Issue 4, Pages: 445-448
https://doi.org/10.2298/HEMIND130513068K
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Determination of ionophore coccidiostat salinomycine in premixes and poultry feeding stuffs by liquid chromatography after post-column derivatisation
Kostadinović Ljiljana M. (Institut za prehrambene tehnologije, Novi Sad)
Kormanjoš Šandor M. (Institut za prehrambene tehnologije, Novi Sad)
Ružičić Lazar N. (Megatrend univerzitet, Fakultet za biofarming, Bačka Topola)
Dozet Gordana K. (Megatrend univerzitet, Fakultet za biofarming, Bačka Topola)
Coccidiosis is a common parasitic disease of broiler chickens caused by
single-celled protozoan parasites of the genus Eimeria which are commonly
referred to as coccidian. This is an infective disease of the digestive tract
which is most frequent with poultry, causing a decrease in daily increment,
prolonged fattening, poorer skin pigmentation, slower feed conversion and
increased mortality. The disease is caused by Protozoas from the genera of
Eimeria, Isospora and Cryptospora, and it is manifested by damaging the
intestine epithelial cells, less frequently the bile duct and renal tubuli.
Coccidiosis is traditionally controlled by chemotherapy. There are many
anticoccidial preparations which are used in the prevention of coccidiosis.
We are chose a polyether monocarboxylic acid - salinomycine. Salinomycin is
monovalent carboxyl-polyether ionophores. Salinomycin, produced by
Streptomyces albus, destroys the cell membranes and causes their lysis.
Salinomycin and other ionophoric antibiotics combine with a number of mono
and divalent cations and in the form of bi-complexes make it possible to
transfer metal ions through lipid hydrophobic membrane, and when they are
added to diet, they change bioavailability, gut uptake and absorption and
reserves of nutrient tissues. In this paper has been developed and validated
process of Liquid chromatography determination of ionophoric coccidiostat
salinomycin with UV spectrophotometric detection and post-column
derivatisation with dimethylaminobenzaldehyde (DMAB). The method is based on
extraction of salinomycin in animal feed samples using mixture
acetonitrile-water (80:20, v/v) and purification of extracts obtained by the
filter 0,2 μm Acrodisc® PSF. The relative standard deviation (RSD) for
reproducibility and accuracy varied from 2,4 to 8,8% and from 2,6% to 8,8%,
respectively and the values for the relative recovery rate ranged from 89 to
98%. The limit of detection (LOD) and limit of quantification (LOQ) were
estimated to be below 47 μg/kg and 71 μg/kg, respectively. Based on this
results, it is concluded that the described method is accurate, precise,
selective and reproducible and can be applied for determination of
salinomycine in feeds and premixes for poultry.
Keywords: salinomycine, liquid chromatography, post-column derivatisation, method validation, feed for poultry