Genetika 2019 Volume 51, Issue 1, Pages: 335-345
https://doi.org/10.2298/GENSR1901335R
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Genetic diversity and structure of autochthonous cattle breeds from Bosnia and Herzegovina based on microsatellites
Rogić Biljana (University of Banja Luka, Faculty of Agriculture, Banja Luka, Republic of Srpska, B&H)
Važić Božo (University of Banja Luka, Faculty of Agriculture, Banja Luka, Republic of Srpska, B&H)
Đan Mihajla (Faculty of Sciences, Department of Biology and Ecology, Novi Sad)
Stamenković-Radak Marina (Faculty of Biology, Belgrade)
Genotype data from 21 microsatellites were used to evaluate genetic diversity
and relationships of Gatacko cattle and Busha breeds. A total of 100 animals
included in the study were divided into three groups: Gatacko cattle (GC),
Busha from eastern Herzegovina (BEH) and Busha from western Herzegovina
(BWH). Hardy-Weinberg equilibrium was observed for all loci in all groups,
except for single locus (INRA35) in group of Busha from western Herzegovina.
A total number of 166 alleles were detected in a total sample. The average
number of alleles per locus was 7.9. The highest observed mean
heterozygosity (0.688) was found in the Gatacko cattle and the lowest
(0.634) in Busha from western Herzegovina. A pairwise population matrix of
Fst values among analyzed groups showed significantly high genetic
differentiation among all three groups: Gatacko cattle and Busha from
western Herzegovina, Gatacko cattle and Busha from eastern Herzegovina and
Busha from western and Busha from eastern Herzegovina. This report
represents the first genetic characterization of Gatacko cattle and Busha
cattle, traditional cattle breed from Bosnia and Herzegovina. The obtained
results are valuable for the future development of conservation and
management strategies for these cattle breeds.
Keywords: genetic diversity and structure, microsatellites, Gatacko cattle, Busha