Genetika 2018 Volume 50, Issue 2, Pages: 519-532
https://doi.org/10.2298/GENSR1802519G
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Molecular taxonomy and phylogenetics of Daedaleopsis confragosa (Bolt.: Fr.) J. Schröt. From wild cherry in Serbia
Galović Vladislava (Institute of Lowland Forestry and Environment, Novi Sad)
Marković Miroslav (Institute of Lowland Forestry and Environment, Novi Sad)
Pap Predrag (Institute of Lowland Forestry and Environment, Novi Sad)
Mulett Martin (Centre for Forestry and Climate Change (Centre for Forestry and Climate change Forest Research, Alice Holt Lodge Farnham Surrey, UK)
Rakić Milana (Faculty of Sciences, Department of Biology and Ecology, Novi Sad)
Vasiljević Aleksandar (Gljivarsko društvo Novi Sad, Novi Sad)
Pekeč Saša (Institute of Lowland Forestry and Environment, Novi Sad)
Daedaleopsis spp., a lignicolous fungus causes of white rot on wild cherry
and other broadleaved species and makes economic losses in Serbian forestry.
The paper presents results of two morphologically distinct fungi
Daedaleopsis confragosa and Daedaleopsis tricolor isolated from native
populations of wild cherry (Prunus avium L.) found in the sites of Protected
Forests of Serbia. Morphological appearance of D. tricolor was found more
abundant in comparison to D. confragosa species. Samples from Serbia were
analysed using morphometric and molecular tools and compared with isolates
from United Kingdom and published sequences from Sweden, Austria, Hungary,
Germany, Canada, France, USA and Czech Republic to give the taxonomic
insight and their genetic relatedness using fungal barcoding region ITS
rDNA. Results from BLAST search confirmed morphology of the isolates to
their taxonomic affiliation as D. confragosa while sequence analysis showed
mutations at several polymorphic positions that indicates genetic divergence
to D. tricolor. Phylogenetic analysis presents narrow genetic relations of
Serbian isolates with the one from United Kingdom while distinctness from
other countries investigated specimens. Future work needs variable regions
for both species to be amplified in order to evaluate species boundaries or
employing NGS technologies in more detailed sequence analyses.
Keywords: Prunus avium, Daedaleopsis confragosa, ITS rDNA, molecular taxonomy, phylogenetics