Chemical Industry and Chemical Engineering Quarterly 2012 Volume 18, Issue 3, Pages: 399-405
https://doi.org/10.2298/CICEQ111118015Y
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Determination of human growth hormone in pure and pharmaceutical dosage form by spectrofluorometry and high performance liquid chromatography
Yilmaz Bilal (Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey)
Kadioglu Yucel (Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey)
Meral Kadem (Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey)
Onganer Yavuz (Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey)
In this study, a new and rapid spectrofluorometry and high performance liquid
chromatography (HPLC) methods were developed for determination of human
growth hormone in pure and pharmaceutical dosage form. The solvent system,
wavelength of detection and chromatographic conditions were optimized in
order to maximize the sensitivity of both the proposed methods. The linearity
was established over the concentration range of 1.25-50μg mL-1 for
spectrofluorometry and 10-75μg mL-1 for HPLC method. The intra- and inter-day
relative standard deviation (RSD) was less than 8.46 and 5.98% for
spectrofluorometry and HPLC, respectively. Limits of quantitation were
determined as 0.075 and 7.5μg mL-1 for spectrofluorometry and HPLC,
respectively. No interference was found from tablet excipients at the
selected assay conditions. The methods were applied for the quality control
of commercial growth hormone dosage form to quantify the drug and to check
the formulation content uniformity.
Keywords: growth hormone, spectrofluorometry, HPLC, validation