doiSerbia

Separation and lipophilicity of some new steroid derivatives in normal- and reversed-phase high performance liquid chromatography

Ačanski Marijana M. (Faculty of Technology, Department of Applied and Engineering Chemistry, Novi Sad)
Vujuć Đura N. (Faculty of Technology, Department of Applied and Engineering Chemistry, Novi Sad)
Jovanović-Šanta Suzana (Faculty of Science, Department of Chemistry, Biochemistry and Environmental protection, Novi Sad)

The separation ability and retention of normal and reversed phase HPLC with, respectively, three non-polar and two polar mobile phases, have been studied by measuring the retention constants of a series of newly synthesized estrone derivatives. The separation ability and retention are discussed in terms of the nature of the solute, eluent and stationary phase. Good correlation was found between the retention constants log k0 of newly synthesized estrone derivatives obtained on C-18 column and log P calculated by different methods.

Keywords: column liquid chromatography, steroids, retention behaviour, correlation analysis