Archives of Biological Sciences 2015 Volume 67, Issue 1, Pages: 261-273
https://doi.org/10.2298/ABS140307036E
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Toll-like receptors (TLR) 2, 3, and 4 gene polymorphisms in critically ill patients
Elkilany Atia (Faculty of Biology, Belgrade)
Zeljić Katarina (Faculty of Biology, Belgrade + Military Medical Academy, Institute for Medical Research, Belgrade)
Surbatović Maja (Military Medical Academy, Clinic of Anesthesiology and Intensive Therapy, Belgrade + University of Defense, Military Medical Academy, Faculty of Medicine, Belgrade)
Đorđević Dragan (Military Medical Academy, Clinic of Anesthesiology and Intensive Therapy, Belgrade + University of Defense, Military Medical Academy, Faculty of Medicine, Belgrade)
Magić Zvonko (Military Medical Academy, Clinic of Anesthesiology and Intensive Therapy, Belgrade + University of Defense, Military Medical Academy, Faculty of Medicine, Belgrade)
Božić Biljana (Faculty of Biology, Belgrade + Military Medical Academy, Institute for Medical Research, Belgrade)
Considering that TLR2, TLR3 and TLR4 play very important roles in
inflammatory processes, the question arises whether the presence of
polymorphisms in these genes is associated with susceptibility to sepsis. The
aim of this study was to examine the association of TLR2, TLR3 and TLR4
polymorphisms with clinical characteristics and outcome of critically ill
patients. A follow-up study was conducted on 121 critically ill Caucasian
Serbian patients. Five polymorphisms in TLRs, TLR2 (rs5743708), TLR3
(rs3775291, rs5743312) and TLR4 (rs4986790, rs4986791) were genotyped by
real-time PCR. Investigated polymorphisms in TLR2 and TLR4 were not
associated with the clinical characteristics and outcome of critically ill
patients. TLR3 rs3775291 polymorphism was associated with patient’s outcome
(p=0.018). Patients with sepsis and a TLR3 rs3775291-mutated genotype had a
four-fold higher mortality rate compared to wild type and heterozygous
carriers. Multivariate regression analysis showed that age, sex and TLR3
rs3775291 polymorphism are independent variables of the outcome of critically
ill patients. For the first time, our preliminary findings indicate the role
of TLR3 with MyD88 independent signaling and its polymorphism (TLR3
rs3775291) in sepsis and survival in critically ill Serbian patients.
Keywords: TLR polymorphisms, critically ill patients, sepsis, trauma