doiSerbia

The influence of increased rearing density on medial protocerebral neurosecretory neurons of Lymantria dispar L. caterpillars

Ilijin Larisa (Odsek za fiziologiju insekata i biohemiju, Institut za biološka istraživanja 'Siniša Stanković', Beograd)
Perić-Mataruga Vesna (Odsek za fiziologiju insekata i biohemiju, Institut za biološka istraživanja 'Siniša Stanković', Beograd)
Radojičić R. (Institut za Fiziologiju i biohemiju, Biološki fakultet, Beograd)
Vlahović Milena (Odsek za fiziologiju insekata i biohemiju, Institut za biološka istraživanja 'Siniša Stanković', Beograd)
Mrdaković Marija (Odsek za fiziologiju insekata i biohemiju, Institut za biološka istraživanja 'Siniša Stanković', Beograd)
Mirčić D. (Odsek za fiziologiju insekata i biohemiju, Institut za biološka istraživanja 'Siniša Stanković', Beograd)
Lazarević Jelica (Odsek za fiziologiju insekata i biohemiju, Institut za biološka istraživanja 'Siniša Stanković', Beograd)

Morphometric changes of A1, A1' and A2 protocerebral dorsomedial neurosecretory neurons, total brain protein content and brain protein profiles were analyzed in 4th instar Lymantria dispar larvae under elevated rearing density, i.e. under intense stress when 5 larvae were kept in a petri dish (V = 80 ml), less intense stress when 5 larvae were kept in a plastic cup (V = 300 ml). In the control samples the larvae were reared in isolated conditions. Protein pattern changes in the brain were observed. Proteins with the following molecular masses: 30, 14, 10 and 3.4-2.5 kD were detected in the experimental groups. The size and cytological characteristics of protocerebral dorsomedial neurosecretory neurons were changed under elevated rearing density.

Keywords: Rearing density, brain protein content, brain protein pattern, medial neurosecretory neurons