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Regenerative Medicine

Negative pressure wound therapy promotes wound healing by suppressing macrophage inflammation in diabetic ulcers

    Haichen Song

    Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China

    ,
    Yu Xu

    *Author for correspondence: Tel.: +86 027 8804 1911;

    E-mail Address: xuyu1026@yeah.net

    Department of Otolaryngology Head & Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China

    ,
    Wenchuan Chang

    Department of Otolaryngology Head & Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China

    ,
    Junli Zhuang

    Department of Vascular Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China

    &
    Xiaowei Wu

    Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China

    Published Online:https://doi.org/10.2217/rme-2020-0050

    Aim: This work aims to explore the biological role of negative pressure wound therapy (NPWT) in the treatment of diabetic ulcer. Materials & methods: Full-thickness skin defects were created in diabetic (db/db) and non diabetic (db/m) mice to create wound models. The mice were received NPWT or rapamycin injection. Mouse macrophage cells (Raw264.7) were treated with lipopolysaccharide to induce inflammatory response, and then received negative pressure treatment. We observed the wound healing of mice and examined gene and protein expression and CD68+ macrophage levels. Results: NPWT notably enhanced the wound closure ratio, and inhibited the LC3-II/LC3-I ratio and Beclin-1 expression in diabetes mellitus (DM) mice. NPWT decreased CD68+ macrophage levels in wound tissues of DM mice. The influence conferred by NPWT was abolished by rapamycin treatment. Negative pressure repressed the LC3-II/LC3-I ratio and the expression of Beclin-1, TNF-α, IL-6 and IL-1β in the Raw264.7 cells. Conclusion: NPWT promotes wound healing by suppressing autophagy and macrophage inflammation in DM.

    Graphical abstract

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