Research Article

Bovine serum albumin-mediated synthesis and quorum sensing inhibitory properties of Ag–Ag₂S nanoparticles

Department of Chemistry, Hacettepe University, Beytepe, Ankara, 06800, Turkey

Faculty of Medicine, Department of Immunology, Niğde Ömer Halisdemir University, Niğde, 51240, Turkey

School of Engineering Sciences in Chemistry, Biotechnology & Health, Department of Chemistry, Division of Glycoscience, KTH Royal Institute of Technology, Stockholm, SE-10691, Sweden

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Hossein Rahimi

Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, 45139-56184, Iran

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Hossein Danafar

Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, 45139-56184, Iran

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Murat Barsbay

**Author for correspondence:

E-mail Address: mbarsbay@hacettepe.edu.tr

Department of Chemistry, Hacettepe University, Beytepe, Ankara, 06800, Turkey

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Ali Sharafi

*Author for correspondence:

E-mail Address: sharafi.a@gmail.com

Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, 45139-56184, Iran

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Published Online:28 Feb 2023https://doi.org/10.2217/nnm-2022-0203

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Abstract

Aim: Quorum sensing (QS) is a density-dependent chemical process of cell-to-cell communication in which certain signals are activated, leading to the coordination of pathogenic behaviors and the regulation of virulence in bacteria. Inhibition of QS can prevent biofilm formation and reduce virulence behaviors of bacteria. Herein, bovine serum albumin (BSA)-coated silver nanoparticles (NPs) (Ag–Ag₂S@BSA NPs) were synthesized and studied as an anti-QS agent. Materials & methods: Ag–Ag₂S NPs prepared through a BSA-mediated biomineralization process under ambient aqueous conditions and their physicochemical properties were characterized. The anti-QS activity of the resulting BSA-coated NPs (Ag–Ag₂S@BSA NPs) was investigated for the first time. Results & conclusion: The result confirmed the potential of Ag–Ag₂S@BSA NPs as novel and useful therapeutic tools for antibacterial purposes.

Plain language summary

The overuse of antibiotics has caused the development of drug-resistant strains of bacteria. Nowadays, drug resistance is one of the main challenges in the treatment and prevention of microbial diseases. In general, many bacterial species communicate with each other through a population-dependent mechanism called quorum sensing to control their physiological activities. Therefore, inhibiting the quorum sensing mechanism is an attractive alternative to antibiotics and also reduces the risk of drug resistance, and this can be achieved by nanoparticles (NPs). Among these nanostructures, Ag NPs have received particular attention due to their antimicrobial, antibiofilm, anticancer and antioxidant properties. Herein, we report a method that applies a BSA-guided green and one-pot approach for achieving Ag–Ag₂S NPs as potential antibacterial agents.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 17, No. 28

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History

Received 7 August 2022

Accepted 19 December 2022

Published online 28 February 2023

Published in print December 2022

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Financial & competing interests disclosure

This work was supported by the Deputy of Research of Zanjan University of Medical Sciences (Grant No: A-12-848-28, Ethical code: IR.ZUMS.REC.1399.379). M Barsbay acknowledges the financial support of the of the International Atomic Energy Agency (IAEA) under coordinated research project F22072 (IAEA research contract no. 23498) The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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Bovine serum albumin-mediated synthesis and quorum sensing inhibitory properties of Ag–Ag2S nanoparticles

Abstract

Plain language summary

References

Bovine serum albumin-mediated synthesis and quorum sensing inhibitory properties of Ag–Ag₂S nanoparticles