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Research Article

Maternal obesity is associated with a sex-specific epigenetic programming in human neonatal monocytes

    Fabián Vega-Tapia

    Division of Pediatrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    ,
    Rocío Artigas

    Core Biodata, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    Advanced Center for Chronic Diseases (ACCDiS), Santiago, Chile

    ,
    Cherie Hernández

    Department of Obstetrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    ,
    Ricardo Uauy

    Division of Pediatrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    ,
    Paola Casanello

    Division of Pediatrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    Department of Obstetrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    ,
    Bernardo J Krause

    *Author for correspondence:

    E-mail Address: bernardo.krause@uoh.cl

    Instituto de Ciencias de la Salud, University of O’Higgins, Rancagua, Chile

    &
    Jose A Castro-Rodriguez

    **Author for correspondence:

    E-mail Address: jacastro17@hotmail.com

    Division of Pediatrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    Published Online:https://doi.org/10.2217/epi-2020-0098

    Aim: To determine changes in global DNA methylation in monocytes from neonates of women with obesity, as markers of an immune programming resulting from maternal obesity. Materials & methods: Cord blood monocytes were obtained from neonates born to women with obesity and normal weight, genome-wide differentially methylated CpGs were determined using an Infinium MethylationEPIC-BeadChip (850K). Results: No clustering of samples according to maternal BMI was observed, but sex-specific analysis revealed 71,728 differentially methylated CpGs in female neonates from women with obesity (p < 0.01). DAVID analysis showed increased methylation levels within genes involved in the innate immune response and inflammation. Conclusion: Maternal obesity induces, in a sex-specific manner, an epigenetic programming of monocytes that could contribute to disease later in life.

    Clinical trial registry: This study is registered in ClinicalTrials.gov NCT02903134.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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