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Regenerative Medicine

Recent advances in cell-laden 3D bioprinting: materials, technologies and applications

    Jie Huang

    CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech & Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

    ,
    Han Fu

    CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech & Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

    State Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China

    ,
    Chong Li

    CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech & Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

    ,
    Jianwu Dai

    *Author for correspondence:

    E-mail Address: jwdai@genetics.ac.cn

    CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech & Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

    &
    Zhijun Zhang

    **Author for correspondence:

    E-mail Address: zjzhang2007@sinano.ac.cn

    CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech & Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

    Published Online:https://doi.org/10.2217/3dp-2017-0010

    Fabrication of 3D scaffolds with patient-specific designs, high structural and component complexity, and rapid on-demand production at a low-cost by printing technique has attracted ever-increasing interests in tissue engineering. Cell-laden 3D bioprinting offers good prospects for future organ transplantation. Compared with nonbiological 3D printing, cell-laden 3D bioprinting involves more complex factors, including the choice of printing materials, the strategy of gelling, cell viability and technical challenges. Although cell-populated 3D bioprinting has so many complex factors, it has proven to be a useful and exciting tool with wide potential applications in regenerative medicine to generate a variety of transplantable tissues. In this review, we first overview the bioprinting materials, gelling strategies and some major applications of cell-laden 3D bioprinting, with main focus on the recent advances and current challenges of the field. Finally, we propose some future directions of the cell-populated 3D bioprinting in tissue engineering and regenerative medicine.

    In this review, we first overview the bioprinting materials, gelling strategies and some major applications of cell-populated 3D bioprinting, with main focus on the recent advances and current challenges of the field. Finally, we propose some future directions of the cell-laden 3D bioprinting in tissue engineering and regenerative medicine.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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