The treatment of furcation defects is a complex and difficult task that may compromise the success of periodontal therapy. Here we report a new clinical treatment of a Class II furcation defect using an autogenous bone graft associated with a buccal fat pad (BFP) used as a membrane. The surgical treatment was performed following initial periodontal therapy. Post-operative follow-up appointments were performed at 3, 7, and 12 months. Clinically, after 3 and 7 months, a reduction in probing depth without bleeding on probing and an increase in vertical and horizontal clinical attachment level were observed. After 7 post-operative months, an increase in keratinized gingiva was observed. Radiographically, a significant improvement was noted, with the furcation defect almost completely closed. These results could also be observed after 12 postoperative months. It can be concluded that the combined use of autogenous bone graft and a BFP yielded clinically favorable outcome in the treatment of a mandibular Class II furcation defect.