A comparative investigation on efficiency of bacteriophage lambda and M13 based vectors for delivering and expression of transgene in eukaryote cells

Abedheydari, Elham; Khalaj-kondori, Mohammad; Hosseinpour-Faizi, Mohammad-Ali; Kosari-Nasab, Morteza

doi:10.22067/jcmr.v6i2.37598

Document Type : Research Articles

Authors

University of Tabriz

https://doi.org/10.22067/jcmr.v6i2.37598

Abstract

Gene delivery might be affected by several tribulations based on carrier/vector applied. Bacteriophages lambda and M13 have different genome conformations; linear double-stranded and circular single-stranded respectively. Therefore, it might be expected that these two common classes of gene delivery vehicles will have different capacity for gene delivery and expression in eukaryote cells. To address the possible effects of linear double-stranded and circular single-stranded genome conformations of bacteriophages lambda and M13 on the transgene expression, the transfection efficacy of two vectors based on lambda and M13 were compared in AGS cell line. The GFP encoding sequence was inserted into the Lambda ZAP-CMV XR vector which resulted in λ-ZAP-CMV-GFP construct. The construct was then in vitro packaged using Gigapack® III Gold packaging extract and λ-GFP phage particles were obtained. The λ-GFP phage particles were then used for in vivo excisioning which resulted in M13-CMV-Script-GFP construct. 1011 copy of λ-ZAP-CMV-GFP or M13-CMV-Script-GFP constructs were transfected into AGS cells using lipofectamine 2000. Transfection efficiencies were analyzed by FACS. Results showed that linear double-stranded λ-ZAP-CMV-GFP was efficient than single-stranded form of M13-CMV-Script-GFP while its double-stranded form was efficient than the linear double-stranded λ-ZAP-CMV-GFP construct for transgene delivery and expression. Moreover the GFP signals resulted from transfections by single-stranded form of M13-CMV-Script-GFP construct faded more quickly in comparison to others. These findings highlight that genome conformation of gene carriers might be an important factor when seeking for an appropriate gene carrier/vehicle.

Keywords

20.1001.1.20089147.2014.6.2.7.3

References

Burg M. A., Jensen-Pergakes K., Gonzalez A. M., Ravey P., Baird A. and Larocca D. (2002) Enhanced phagemid particle gene transfer in camptothecin-treated carcinoma cells. Cancer Researc 62:4, 977-81.

Calendar R. and Abedon S. T. (2005) The bacteriophages. Oxford University Press. UK.

Catherine D., Clark J., March J. B. (2004) Bacteriophage lambda is a highly stable DNA vaccine delivery vehicle. Vaccine. 22: 2413-9.

Di Giovine M., Salone B., Martina Y., Amati V., Zambruno G., Cundari E. and Saggio I. (2001) Binding properties, cell delivery, and gene transfer of adenoviral penton base displaying bacteriophage. Virology 282: 102-12.

Kaiser A. (1966) On the internal structure of bacteriophage lambda. J Gen Physiol 49: 171-8.

Khalaj-Kondori M., Sadeghizadeh M., Behmanesh M., Saggio I. and Monaci P. (2011) Chemical coupling as a potent strategy for preparation of targeted bacteriophage-derived gene nanocarriers into eukaryotic cells. Journal of Gene Medicine 13: 622–31.

Khalaj-kondori M., Sadeghizadeh M. and Behmanesh M. (2010) Bacteriophage lambda-mediated gene transfer into human AGS cell line. ICBEE 176-9.

Kim A., Shin T-H., Shin S-M., Pham C. D., Choi D-K., Kwon M-H. and Kim Y-S. (2012) Cellular internalization mechanism and intracellular trafficking of filamentous M13 phages displaying a cell-penetrating transbody and TAT peptide. PloS one 7: e51813.

Lambda ZAP ® -CMV XR Library Construction Kit. In: Stratagene (2007) editor. United States and Canada, Stratagene.

Lankes H.A., Zanghi C.N., Santos K., Capella C. Duke C.M. and Dewhurst S. (2007) In vivo gene delivery and expression by bacteriophage lambda vectors. Journal of Applied Microbiology 102:1337–49.

Larocca D., Kassner P., Witte A., Ladner R., Pierce G. and Baird A. (1999) Gene transfer to mammalian cells using genetically targeted filamentous bacteriophages. FASEB 6: 727–34.

Larocca D., Jensen-Pergakes K., Burg M. and Baird A. (2001) Receptor-targeted gene delivery using multivalent phagemid particles. Molecular Therapy 3: 476–84.

Larocca D. and Baird A. (2001) Receptor-mediated gene transfer by phage-display vectors: applications in functional genomics and gene therapy. Drug Discovery Today 6: 793-801.

Larocca D., Brug M., Jensen-Pergakes K., Prenn R. E., Maria G. A. and Baird A. (2002) Evolving phage vectors for cell targeted gene delivery. Current Pharmacology and biotechnology 3: 45-57.

Larocca D., Jensen-Pergakes K., Burg M. A. and Baird A. (2001) Receptor-targeted gene delivery using multivalent phagemid particles. Molecular Therapy 3: 476-84.

Larocca D., Witte A., Johnson W., Pierce G. F. and Baird A. (1998) Targeting bacteriophage to mammalian cell surface receptors for gene delivery. Human Gene Therapy 9: 2393-9.

Larocca D., Kassner P. D., Witte A., Ladner R. C., Pierce G. F. and Baird A. (1999) Gene transfer to mammalian cells using genetically targeted filamentous bacteriophage. FASEB Journal 13: 727-34.

Olofsson L., Ankarloo J., Andersson P. O. and Nicholls I. A. (2001) Filamentous bacteriophage stability in non-aqueous media. ChemBiol 8: 661-71.

Petrenko V., Smith G., Gong X. and Quinn T. (1996) A library of organic landscapes on filamentous phage. Protein Engineering 9: 797-801.

Piersanti S., Cherubini G., Martina Y., Salone B., Avitabile D., Grosso F. et al. (2004) Mammalian cell transduction and internalization properties of lambda phage displaying the full-length adenoviral penton base or its central domain. Journal of Molecular Medicine 82: 467–76.

Poul M. A. and Marks J. D. (1999) Targeted gene delivery to mammalian cells by filamentous Bacteriophage. Molecular Biology 288: 203-11.

Prieto Y. and Sanchez O. (2007) Self-complementary sequences induce the formation of double-stranded filamentous phages. BiochimBiophysActa 1770: 1081-4.

Sapinoro R., Volcy K., Rodrigo W., Schlesinger J. J. and Dewhurst S. (2008) Fc receptor-mediated, antibody-dependent enhancement of bacteriophage lambda-mediated gene transfer in mammalian cells. Virology 373: 274-86.

Soboleski M. R., Oaks J. and Halford W. P. (2005) Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells. FASEB Journal 19: 440-2.

Akuta T., Eguchi A., Okuyama H., Senda T., Inokuchi H., Suzuki Y., et al. (2002) Enhancement of phage-mediated gene transfer by nuclear localization signal. Biochemistry and Biophysic Research Communication 297: 779–86.

Urbanelli L., Ronchini C., Fontana L., Menard S., Orlandi R. and Monaci P. (2001) Targeted gene transduction of mammalian cells expressing the HER2/neu receptor by filamentous phage. Journal of Molecular Biology 13: 965-76.

Volcy K. and Dewhurst S. (2009) Proteasome inhibitors enhance bacteriophage lambda (λ) mediated gene transfer in mammalian cells. Virology 384: 77-87.

Zanghi C. N., Lankes H. A., Bradel-Tretheway B., Wegman J. and Dewhurst S. (2005) A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda. Nucleic Acids Research 33: e160-e.

Name *

Email Address *

Affiliation *

Comments *

Security Code *

Journal of Cell and Molecular Research

A comparative investigation on efficiency of bacteriophage lambda and M13 based vectors for delivering and expression of transgene in eukaryote cells

References

References

Send comment about this article

Volume 6, Issue 2 - Serial Number 12
January 2014
Pages 69-75

A comparative investigation on efficiency of bacteriophage lambda and M13 based vectors for delivering and expression of transgene in eukaryote cells

References

References

Send comment about this article

Volume 6, Issue 2 - Serial Number 12January 2014Pages 69-75

Volume 6, Issue 2 - Serial Number 12
January 2014
Pages 69-75