초록

Purpose: The purpose of this study was to compare and quantify the expression of macrophage colony-stimulationg factor (M-CSF), receptor activator of nuclear factor kappa-B (RANK) and osteoprotegerin (OPG) that the related to bone resorption in the gingival tissues of patients with type 2 diabetes mellitus (DM) and healthy adults with chronic periodontitis. Materials and Methods: Depending on the patient's systemic condition and clinical criteria of gingiva, each gingival sample was divided into three groups. Group 1 (n=12) is clinically healthy gingiva obtained from systemically healthy 12 patients. Group 2 (n=12) is infl amed gingiva from patients with chronic periodontitis. Group 3 (n=12) is infl amed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The relative quantifi cations of M-CSF, RANK and OPG were performed with a densitometer. Results: The expression levels of M-CSF signifi cantly increased in group 3 as compared to group 1 (p<0.05). The expression levels of RANK were signifi cantly increased in group 3 as compared to group 1 and 2. And In the group 2, the levels of RANK were signifi cantly increased as compared to group 1 (p<0.05). The expression levels of OPG were signifi cantly increased in the group 3 as compared to group 1 and 2 (p<0.05). Conclusion: In conclusion, It can be assumed that M-CSF, RANK and OPG may be involved in the progression of periodontal inflammation associated to type 2 DM. This study demonstrated that the expression levels of RANK and OPG might be infl ammatory markers in periodontal infl amed tissue.

키워드

Diabetes mellitus, Macrophage colony-stimulating factor, Osteoprotegerin, Periodontal disease, RANK

참고문헌(40)open

  1. [학술지] 백영란 / 2007 / 만성 치주염 환자의 치은 조직에서 RANK 및 RANKL의 발현 / Journal of Periodontal & Implant Science 37 (4) : 849 ~ 857

  2. [학술지] Bickel M / 2001 / Cytokine gene expression in chronic periodontitis / J Clin Periodontol 28 : 840 ~ 847

  3. [학술지] 채영미 / 2009 / Upregulation of smpd3 via BMP2 stimulation and Runx2 / BMB reports 42 (2) : 86 ~ 90

  4. [학술지] Cho JY / 2000 / Expression and activity of human Na+/Isymporter in human glioma cells by adenovirus-mediated gene delivery / Gene Ther 7 : 740 ~ 749

  5. [학술지] Costa PP / 2010 / Salivary interleukin-6, matrix metalloproteinase-8, and osteoprotegerin in patients with periodontitis and diabetes / J Periodontol 81 : 384 ~ 391

  6. [학술지] Crotti T / 2003 / Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis / J Periodontal Res 38 : 380 ~ 387

  7. [학술지] Emrich LJ / 1991 / Periodontal disease in noninsulin- dependent diabetes mellitus / J Periodontol 62 : 123 ~ 131

  8. [학술지] Fixe P / 1998 / M-CSF: haematopoietic growth factor or infl ammatory cytokine? / Cytokine 10 : 32 ~ 37

  9. [학술지] Grossi SG / 1994 / Assessment of risk for periodontal disease. I. Risk indicators for attachment loss / J Periodontol 65 : 260 ~ 267

  10. [학술지] Hormdee D / 2005 / Protein kinase-A-dependent osteoprotegerin production on interleukin-1 stimulation in human gingival fibroblasts is distinct from periodontal ligament fibroblasts / Clin Exp Immunol 142 : 490 ~ 497

  11. [학술지] Hugoson A / 1989 / Periodontal conditions in insulin-dependent diabetics / J Clin Periodontol 16 : 215 ~ 223

  12. [학술지] Iacopino AM / 2001 / Periodontitis and diabetes interrelationships: role of infl ammation / Ann Periodontol 6 : 125 ~ 137

  13. [학술지] 주상돈 / 2007 / 단순 만성 치주염 환자 및 2형 당뇨병환자의 만성 치주염 치은조직에서 염증성 매개인자의 발현 양상 비교 / Journal of Periodontal & Implant Science 37 (5) : 353 ~ 369

  14. [학술지] Katagiri T / 2002 / Regulatory mechanisms of osteoblast and osteoclast diff erentiation / Oral Dis 8 : 147 ~ 159

  15. [학술지] Kawano Y / 2004 / The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells / Am J Reprod Immunol 52 : 124 ~ 128

  16. [학술지] Lacey DL / 1998 / Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation / Cell 93 : 165 ~ 176

  17. [학술지] Liu D / 2003 / Expression of RANKL and OPG mRNA in periodontal disease: possible involvement in bone destruction / Int J Mol Med 11 : 17 ~ 21

  18. [학술지] McCauley LK / 2002 / Mediators of periodontal osseous destruction and remodeling: principles and implications for diagnosis and therapy / J Periodontol 73 : 1377 ~ 1391

  19. [학술지] Mizuno N / 2005 / Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bonerelated and enamel proteins / Cell Biol Int 29 : 111 ~ 117

  20. [학술지] Mühlemann HR / 1971 / Gingival sulcus bleeding--a leading symptom in initial gingivitis / Helv Odontol Acta 15 : 107 ~ 113

  21. [학술지] Nabipour I / 2010 / Osteoprotegerin in relation to type 2 diabetes mellitus and the metabolic syndrome in postmenopausal women / Metabolism 59 : 742 ~ 747

  22. [학술지] Nagasawa T / 2007 / Roles of receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin in periodontal health and disease / Periodontol 2000 43 : 65 ~ 84

  23. [학술지] Page RC / 1991 / The role of infl ammatory mediators in the pathogenesis of periodontal disease / J Periodontal Res 26 (3) : 230 ~ 242

  24. [학술지] 박재완 / 2007 / 단순 만성 치주염 환자 및 2형 당뇨병환자의 만성 치주염 치은조직에서 IL-6, elastase 및 α1-PI의 발현 양상 비교 / Journal of Periodontal & Implant Science 37 (5) : 325 ~ 338

  25. [학술지] Pontes Andersen CC / 2007 / Relationship between periodontitis and diabetes: lessons from rodent studies / J Periodontol 78 : 1264 ~ 1275

  26. [학술지] Rani CS / 2000 / Dental cells express factors that regulate bone resorption / Mol Cell Biol Res Commun 3 : 145 ~ 152

  27. [학술지] Sakata M / 1999 / Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells / J Bone Miner Res 14 : 1486 ~ 1492

  28. [학술지] Salvi GE / 1997 / Inflammatory mediator response as a potential risk marker for periodontal diseases in insulin-dependent diabetes mellitus patients / J Periodontol 68 : 127 ~ 135

  29. [학술지] Simonet WS / 1997 / Osteoprotegerin: a novel secreted protein involved in the regulation of bone density / Cell 89 : 309 ~ 319

  30. [학술지] Suda T / 1992 / Modulation of osteoclast differentiation / Endocr Rev 13 : 66 ~ 80

  31. [학술지] Suda T / 1999 / Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families / Endocr Rev 20 : 345 ~ 357

  32. [학술지] Tanaka S / 1993 / Macrophage colonystimulating factor is indispensable for both proliferation and differentiation of osteoclast progenitors / J Clin Invest 91 : 257 ~ 263

  33. [학술지] Teng YT / 2000 / Functional human T-cell immunity and osteoprotegerin ligand control alveolar bone destruction in periodontal infection / J Clin Invest 106 : R59 ~ R67

  34. [학술지] Theill LE / 2002 / RANK-L and RANK: T cells, bone loss, and mammalian evolution / Annu Rev Immunol 20 : 795 ~ 823

  35. [학술지] Wada T / 2006 / RANKL-RANK signaling in osteoclastogenesis and bone disease / Trends Mol Med 12 : 17 ~ 25

  36. [학술지] Yalda B / 1994 / Diabetes as a modifier of periodontal disease expression / Periodontol 2000 6 : 37 ~ 49

  37. [학술지] Yamazaki H / 2001 / Presence of osteoclast precursors in colonies cloned in the presence of hematopoietic colony-stimulating factors / Exp Hematol 29 : 68 ~ 76

  38. [학술대회] Yasuda H / 1998 / Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL / Proc Natl Acad Sci U S A 95 : 3597 ~ 3602

  39. [학술지] Yasuda H / 1998 / Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro / Endocrinology 139 : 1329 ~ 1337

  40. [학술지] Yaturu S / 2008 / Relationship of elevated osteoprotegerin with insulin resistance, CRP, and TNF-alpha levels in men with type 2 diabetes / Cytokine 44 : 168 ~ 171