Reduction of Genotoxicity of Carbamazepine to Human Lymphocytes by
Pre-treatment with Vitamin B12

Eman   K.   Hendawi; Omar   F.   Khabour; Laith   N.   Al-Eitan; Karem   H.   Alzoubi

Abstract

Background: Carbamazepine (CBZ) is widely used as an anti-epileptic drug. Vitamin B12 has been shown to protect against DNA damage caused by several mutagenic agents.

Objective: This study aimed to investigate the effect of vitamin B12 on CBZ-induced genotoxicity in cultured human lymphocytes.

Methods: Sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) genotoxic assays were utilized to achieve the study objective.

Results: The results showed significantly higher frequencies of CAs and SCEs in the CBZ-treated cultures (12 μg/mL) compared to the control group (P<0.01). The genotoxic effects of CBZ were reduced by pre-treatment of cultures with vitamin B12 (13.5μg/ml, P<0.05). Neither CBZ nor vitamin B-12 showed any effects on mitotic and proliferative indices.

Conclusion: CBZ is genotoxic to lymphocyte cells, and this genotoxicity can be reduced by vitamin B12.

Keywords: Carbamazepine, vitamin B12, sister chromatid exchanges, DNA damage, chromosomal aberrations, genotoxicity, white lymphocytes.

Graphical Abstract

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DOI https://dx.doi.org/10.2174/1874467215666220420135924	Print ISSN 1874-4672
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Current Molecular Pharmacology

Reduction of Genotoxicity of Carbamazepine to Human Lymphocytes by Pre-treatment with Vitamin B12

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