Abstract
A peculiar population of glial cells, Olfactory Ensheathing Cells (OECs), are able to support the continuous neuronal turn-over and sheathe olfactory axons. In vitro, they stimulate axonal growth, as produce several neurotrophic factors (GFs); in vivo they promote remyelination of damaged axons. In this in vitro study, OEC effects on survival of cortical neurons exposed to hypoxia were examined. Rat co-cultures of OECs and cortical neurons were placed both in normal and hypoxic conditions; subsequently cells were analyzed by immunocytochemistry. Furthermore, some neuronal cultures were grown with Glial cell Derived Neurotrophic Factor (GDNF) or basic Fibroblast Growth Factor (bFGF) to tentatively rescue cells from oxygen deprivation. Some cortical neurons grown in both conditions were considered as control cells. Some neuronal cultures were feed with conditioned medium from OECs. We show that both in co-cultures and with GFs-treatment there was an increase of the number of neurons in comparison with control cultures. Moreover, these neurons formed a rich axonal outgrowth. OEC-conditioned media did not affect the cell survival. In hypoxic cultures the neuron number was very low both in controls and in GFs-treated neurons, while in co-cultures and in OEC-conditioned media cultures an increased neuronal survival was observed. These data suggest that OECs promote the survival of neurons in vitro exposed to hypoxia exerting a protective influence. Since some experiments in vivo have shown that injury is often characterized by secondary insults, ischemia or hypoxia, our results suggest that OECs might be considered a possible approach for restoration in injuries.
Keywords: Cell viability, cortical neurons, growth factors, hypoxia, immunocytochemistry, neuroprotective effect, olfactory ensheathing cells.
CNS & Neurological Disorders - Drug Targets
Title:Olfactory Ensheathing Cells Protect Cortical Neuron Cultures Exposed to Hypoxia
Volume: 14 Issue: 1
Author(s): Rosalia Pellitteri, Antonella Russo, Stefania Stanzani and Damiano Zaccheo
Affiliation:
Keywords: Cell viability, cortical neurons, growth factors, hypoxia, immunocytochemistry, neuroprotective effect, olfactory ensheathing cells.
Abstract: A peculiar population of glial cells, Olfactory Ensheathing Cells (OECs), are able to support the continuous neuronal turn-over and sheathe olfactory axons. In vitro, they stimulate axonal growth, as produce several neurotrophic factors (GFs); in vivo they promote remyelination of damaged axons. In this in vitro study, OEC effects on survival of cortical neurons exposed to hypoxia were examined. Rat co-cultures of OECs and cortical neurons were placed both in normal and hypoxic conditions; subsequently cells were analyzed by immunocytochemistry. Furthermore, some neuronal cultures were grown with Glial cell Derived Neurotrophic Factor (GDNF) or basic Fibroblast Growth Factor (bFGF) to tentatively rescue cells from oxygen deprivation. Some cortical neurons grown in both conditions were considered as control cells. Some neuronal cultures were feed with conditioned medium from OECs. We show that both in co-cultures and with GFs-treatment there was an increase of the number of neurons in comparison with control cultures. Moreover, these neurons formed a rich axonal outgrowth. OEC-conditioned media did not affect the cell survival. In hypoxic cultures the neuron number was very low both in controls and in GFs-treated neurons, while in co-cultures and in OEC-conditioned media cultures an increased neuronal survival was observed. These data suggest that OECs promote the survival of neurons in vitro exposed to hypoxia exerting a protective influence. Since some experiments in vivo have shown that injury is often characterized by secondary insults, ischemia or hypoxia, our results suggest that OECs might be considered a possible approach for restoration in injuries.
Export Options
About this article
Cite this article as:
Pellitteri Rosalia, Russo Antonella, Stanzani Stefania and Zaccheo Damiano, Olfactory Ensheathing Cells Protect Cortical Neuron Cultures Exposed to Hypoxia, CNS & Neurological Disorders - Drug Targets 2015; 14 (1) . https://dx.doi.org/10.2174/1871527314666150116121105
DOI https://dx.doi.org/10.2174/1871527314666150116121105 |
Print ISSN 1871-5273 |
Publisher Name Bentham Science Publisher |
Online ISSN 1996-3181 |
Call for Papers in Thematic Issues
Diagnosis and treatment of central nervous system infectious diseases
Infectious diseases of the central nervous system (CNS) can be divided into bacterial, tuberculous, viral, fungal, parasitic infections, etc. Early etiological treatment is often the most crucial means to reduce the mortality rate of patients with central nervous system infections, reduce complications and sequelae, and improve prognosis. The initial clinical ...read more
Techniques of Drug Repurposing: Delivering a new life to Herbs & Drugs
Of late, with the adaptation of innovative approaches and integration of advancements made towards medical sciences as well as the availability of a wide range of tools; several therapeutic challenges are being translated into viable clinical solutions, with a high degree of efficacy, safety, and selectivity. With a better understanding ...read more
Trends and perspectives in the rational management of CNS disorders
Central nervous system (CNS) diseases enforce a significant global health burden, driving ongoing efforts to improve our understanding and effectiveness of therapy. This issue investigates current advances in the discipline, focusing on the understanding as well as therapeutic handling of various CNS diseases. The issue covers a variety of diseases, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Daptomycin: A Review of Properties, Clinical Use, Drug Delivery and Resistance
Mini-Reviews in Medicinal Chemistry Association Between Seizures and Diabetes Mellitus: A Comprehensive Review of Literature
Current Diabetes Reviews Small Neuroscience: The Nanostructure of the Central Nervous System and Emerging Nanotechnology Applications
Current Nanoscience Moxifloxacin-induced Hypoglycemia in a Non-diabetic Patient
Current Drug Safety Amyloid Beta Protein as a Marker or Risk Factor of Alzheimers Disease
Current Alzheimer Research The Potentials of Uncariae Ramulus Cum Uncis for the Treatment of Migraine: Targeting CGRP in the Trigeminovascular System
Current Neuropharmacology Anti-Infective Strategies of the Future: Is there Room for Species-Specific Antibacterial Agents?
Current Pharmaceutical Design Current and Future Therapies Targeting the Immune System in Multiple Sclerosis
Current Pharmaceutical Biotechnology Molecular Dynamics Simulations of Novel Potential Inhibitors for Penicillin Binding Protein 2B of the Resistant 5204 Strain of Streptococcus pneumoniae
Current Computer-Aided Drug Design Immune Restoration Disease: A Consequence of Dysregulated Immune Responses After HAART
Current HIV Research Monoclonal Antibodies in Rare Acquired Thrombotic Disorders: The Paradigms of Paroxysmal Nocturnal Hemoglobinuria, Thrombotic Thrombocytopenic Purpura and Central Retinal Vein Occlusion
Current Vascular Pharmacology Techniques and Methods for In Vivo MRI Monitoring of Exogenous and Endogenous Neural Stem Cell-Mediated Brain Repair
Recent Patents on Regenerative Medicine Activation and Control of CNS Innate Immune Responses in Health and Diseases: A Balancing Act Finely Tuned by Neuroimmune Regulators (NIReg)
CNS & Neurological Disorders - Drug Targets Current Approaches in Antiviral Drug Discovery Against the Flaviviridae Family
Current Pharmaceutical Design Anti-Viral Agents in Neurodegenerative Disorders: New Paradigm for Targeting Alzheimer’s Disease
Recent Patents on Anti-Infective Drug Discovery Genetically Modified Viruses Vaccines by Design
Current Pharmaceutical Biotechnology Subdural Pharmacotherapy for the Treatment of Intractable Focal Neocortical Epilepsy
Drug Delivery Letters Hypertension in Pregnancy: Pathophysiology & Management Strategies
Current Pharmaceutical Design Commentary: (Research Highlights Inflammation, Demyelination and Neurodegeneration: Risky Buddies in Multiple Sclerosis)
CNS & Neurological Disorders - Drug Targets Arboviral Encephalitis and RNAi Treatment
Central Nervous System Agents in Medicinal Chemistry