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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

An Easy Efficient Method of Veterinary Drug Residue Analysis in Raw Milk by RP-HPLC-UV with Application to Raw Milk

Author(s): Lekweiri Haiba Legrae*, Mohamed Fadel Deida, Bah Mohamed Lemine Abdellahi, Mohamed Brahim Elkory, Ibrahima Ndiaye and Jalloul Bouajila*

Volume 16, Issue 7, 2020

Page: [942 - 949] Pages: 8

DOI: 10.2174/1573412915666190416115517

Price: $65

Abstract

Background: This study reports an easy method of a veterinary drug investigation in raw milk, based on QuECHERS extraction followed by RP-HPLC-UV analysis. Use of this benchtop system was motivated by its availability and moderate cost relatively to other sophisticated methods such as LC-MS which are more efficient.

Methods: This developed method has been optimized and then after validation according to EU legislation, it demonstrated good linearity with R²>0.997, acceptable peak resolution within a short time (<9.5 min) and good recovery of the analyzed drugs (OXY, ALZ and IVR, respectively 87.08, 99.02 and 92.01 %). Additionally, we applied the method to the analysis of cow milk, collected in Nouakchott, capital of Mauritania.

Results: The obtained results indicated a mixed level of drug use according to targeted molecules. In 42% of sampled farms, the anti-parasitics IVR and ALZ were detected whereas the antibiotic OXY was detected in 50%.

Conclusion: This investigation shows that 17% of the sampled farms exceeded European standards for IVR drug.

Keywords: Veterinary drug residue, oxytetracycline, ivermectin, albendazole, QuECHERS method, sahel region.

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[1]
Taine-Cheikh, C. le lait dans l'alimentation des nomades de mauritanie, awal (cahiers d'études berbères), numéro spécial 2014, 42, 27-50.
[2]
pesticides et médicaments en santé animale, rencontre interdisciplinaires de technologie, 16-18 février, 1989, bruxelles, liège (belgique). 1989.
[3]
Schelling, E.; Wyss, K.; Béchir, M.; Moto, D.D.; Zinsstag, J. Synergy between public health and veterinary services to deliver human and animal health interventions in rural low income settings. BMJ, 2005, 331(7527), 1264-1267.
[http://dx.doi.org/10.1136/bmj.331.7527.1264] [PMID: 16308393]
[4]
Montavon, A.V; Jean-Richard, M.; Bechir, D.M.; Daugla, M Abdoulaye, R.N.; Bongo Naré, C.; Diguimbaye-Djaibé, I.O.; Alfaroukh, E.; Schelling, K.; Wyss, M.; Tanner, J.; Zinsstag.. La santé des pasteurs mobiles au Sahel – Bilan de 15 années de recherches et développement. TMIH, 2013, 18(9), E1-E10.
[5]
Garba, A.R. Evaluation des pratiques d’utilisation des médicaments vété-rinaires et détermination de la prévalence des résidus d’antibiotiques dans la viande et le lait dans le Gorgol en Mauritanie, Mémoire de Diplôme de Master en Santé Publique vétérinaire, 2012, université Cheikh Anta Diop de Dakar,; Sé-négal, 2012.
[6]
Chung, H.H.; Lee, J.B.; Chung, Y.H.; Lee, K.G. Analysis of sulfonamide and quinolone antibiotic residues in Korean milk using microbial assays and high performance liquid chromatography. Food Chem., 2009, 113, 297-301.
[http://dx.doi.org/10.1016/j.foodchem.2008.07.021]
[7]
Adrian, J.; Pinacho, D.G.; Granier, B.; Diserens, J.M.; SánchezBaeza, F.; Marco, M.P.A. A multianalyte ELISA for immunochemical screening of sulfonamide, fluoroquinolone and ss-lactam antibiotics in milk samples using class-selective bioreceptors. Anal. Bioanal. Chem., 2008, 391(5), 1703-1712.
[http://dx.doi.org/10.1007/s00216-008-2106-9] [PMID: 18483810]
[8]
Moreno-Bondi, M.C.; Marazuela, M.D.; Herranz, S.; Rodriguez, E. An overview of sample preparation procedures for LC-MS multiclass antibiotic determination in environmental and food samples. Anal. Bioanal. Chem., 2009, 395(4), 921-946.
[http://dx.doi.org/10.1007/s00216-009-2920-8] [PMID: 19633833]
[9]
Bogialli, S.; Di Corcia, A. Recent applications of liquid chromatography-mass spectrometry to residue analysis of antimicrobials in food of animal origin. Anal. Bioanal. Chem., 2009, 395(4), 947-966.
[http://dx.doi.org/10.1007/s00216-009-2930-6] [PMID: 19609510]
[10]
Reig, M.; Toldrá, F. Veterinary drug residues in meat: Concerns and rapid methods for detection. Meat Sci., 2008, 78(1-2), 60-67.
[http://dx.doi.org/10.1016/j.meatsci.2007.07.029] [PMID: 22062096]
[11]
Loi No 2010-0042 relative au code d’hygiène, adoptée et promulguée: journal officiel de république islamique de mauritanie, aout,, 2010.
[12]
council regulation (eec) no. 37/2010 of 22 december 2009 on pharmacologically active substances and their classifica-tion regarding maximum residue limits in foodstuffs of ani-mal origin. off. j. eur. communities, 2010, 15(1), 20.1.,
[13]
the code of federal regulations, title 21, food and drugs, available from: http://www.access.gpo.gov/nara/cfr/cfr-tablesearch.html
[14]
commission regulation (eu) 37/2010, on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. off. j. eur. communities. 2010, 15, 1−72.
[15]
codex alimentarius commission maximum residue limits for veterinary drugs in foods updated as at the 35th session of the codex alimentarius commission, 2012.july,
[16]
Sundlof, S.F. veterinary drugs residues: veterinary drugs-general, in: ymotarjemi, encyclopedia of food safety, elsevier inc., mi; usa, 2014, 3, p., , 35-38.
[17]
commission decision of 12 august 2002 implementing council directive 96/23/ec concerning the performance of analytical methods and the interpretation of results (2002/657/ec); , 2014.
[18]
conséquences de la directive européenne 2009/90/ce sur l’estimation des limites de quantification des méthodes d’analyse chimique (rapport final); , 2014.
[19]
Kulik, A.; Szczotkowska, A.; Białecka, W.; Podolska, M.; Kwiatkowska-Puchniarz, B.; Mazurek, A. Determination of active substances in binary mixture antiparasitic veterinary formulations by HPLC. Acta Pol. Pharm., 2011, 68(4), 467-472.
[PMID: 21796928]
[20]
Laurentie, M.; Sanders, P. Résidus de médicaments vétérinaires et temps d’attente dans le lait. Bulletin des Groupements Techniques Vétérinaires, 2002, 15, 197-201.
[21]
Wang, K.; Lin, K.; Huang, X.; Chen, M. A Simple and fast extraction method for the determination of multiclass antibiotics in eggs using LC-MS/MS. J. Agric. Food Chem., 2017, 65(24), 5064-5073.
[http://dx.doi.org/10.1021/acs.jafc.7b01777] [PMID: 28581742]
[22]
Aguilera-Luiz, M.M.; Vidal, J.L.M.; Romero-González, R.; Frenich, A.G. Multi-residue determination of veterinary drugs in milk by ultra-high-pressure liquid chromatography-tandem mass spectrometry. J. Chromatogr. A, 2008, 1205(1-2), 10-16.
[http://dx.doi.org/10.1016/j.chroma.2008.07.066] [PMID: 18752803]
[23]
García-Mayor, M.A.; Garcinuño, R.M.; Fernández-Hernando, P.; Durand-Alegría, J.S. Liquid chromatography-UV diode-array detection method for multi-residue determination of macrolide antibiotics in sheep’s milk. J. Chromatogr. A, 2006, 1122(1-2), 76-83.
[http://dx.doi.org/10.1016/j.chroma.2006.04.019] [PMID: 16682049]
[24]
De Ruyck, H.; De Ridder, H. Determination of tetracycline antibiotics in cow’s milk by liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom., 2007, 21(9), 1511-1520.
[http://dx.doi.org/10.1002/rcm.2991] [PMID: 17394125]
[25]
Yang, S.; Cha, J.; Carlson, K. Trace analysis and occurrence of anhydroerythromycin and tylosin in influent and effluent wastewater by liquid chromatography combined with electrospray tandem mass spectrometry. Anal. Bioanal. Chem., 2006, 385(3), 623-636.
[http://dx.doi.org/10.1007/s00216-006-0416-3] [PMID: 16715282]
[26]
Dasenaki, M.E.; Bletsou, A.A.; Koulis, G.A.; Thomaidis, N.S. Qualitative multiresidue screening method for 143 veterinary drugs and pharmaceuticals in milk and fish tissue using liquid chromatography quadrupole-time-of-flight mass spectrometry. J. Agric. Food Chem., 2015, 63(18), 4493-4508.
[http://dx.doi.org/10.1021/acs.jafc.5b00962] [PMID: 25826150]
[27]
Wittenberg, J.B.; Simon, K.A.; Wong, J.W. Targeted multiresidue analysis of veterinary drugs in milk-based powders using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). J. Agric. Food Chem., 2017, 65(34), 7288-7293.
[http://dx.doi.org/10.1021/acs.jafc.6b05263] [PMID: 28094519]
[28]
Kaufmann, A.; Butcher, P.; Maden, K.; Walker, S.; Widmer, M. Quantification of anthelmintic drug residues in milk and muscle tissues by liquid chromatography coupled to Orbitrap and liquid chromatography coupled to tandem mass spectrometry. Talanta, 2011, 85(2), 991-1000.
[http://dx.doi.org/10.1016/j.talanta.2011.05.009] [PMID: 21726729]
[29]
Zhan, J.; Yu, X.J.; Zhong, Y.Y.; Zhang, Z.T.; Cui, X.M.; Peng, J.F.; Feng, R.; Liu, X.T.; Zhu, Y. Generic and rapid determination of veterinary drug residues and other contaminants in raw milk by ultra-performance liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2012, 906, 48-57.
[http://dx.doi.org/10.1016/j.jchromb.2012.08.018] [PMID: 22959038]
[30]
Romero-González, R.; Aguilera-Luiz, M.M.; Plaza-Bolaños, P.; Frenich, A.G.; Vidal, J.L. Food contaminant analysis at high resolution mass spectrometry: application for the determination of veterinary drugs in milk. J. Chromatogr. A, 2011, 1218(52), 9353-9365.
[http://dx.doi.org/10.1016/j.chroma.2011.10.074] [PMID: 22098928]
[31]
Kinsella, B.; Lehotay, S.J.; Mastovska, K.; Lightfield, A.R.; Furey, A.; Danaher, M. New method for the analysis of flukicide and other anthelmintic residues in bovine milk and liver using liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta, 2009, 637(1-2), 196-207.
[http://dx.doi.org/10.1016/j.aca.2008.10.072] [PMID: 19286030]
[32]
Kinsella, B.; Whelan, M.; Cantwell, H.; McCormack, M.; Furey, A.; Lehotay, S.J.; Danaher, M. A dual validation approach to detect anthelmintic residues in bovine liver over an extended concentration range. Talanta, 2010, 83(1), 14-24.
[http://dx.doi.org/10.1016/j.talanta.2010.08.025] [PMID: 21035637]
[33]
Whelan, M.; Kinsella, B.; Furey, A.; Moloney, M.; Cantwell, H.; Lehotay, S.J.; Danaher, M. Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatographytandem mass spectrometry with rapid polarity switching. J. Chromatogr. A, 2010, 1217(27), 4612-4622.
[http://dx.doi.org/10.1016/j.chroma.2010.05.007] [PMID: 20564781]
[34]
Ortelli, D.; Cognard, E.; Jan, P.; Edder, P. Comprehensive fast multiresidue screening of 150 veterinary drugs in milk by ultraperformance liquid chromatography coupled to time of flight mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2009, 877(23), 2363-2374.
[http://dx.doi.org/10.1016/j.jchromb.2009.03.006] [PMID: 19328746]
[35]
Grimalt, S.; Dehouck, P. Review of analytical methods for the determination of pesticide residues in grapes. J. Chromatogr. A, 2016, 1433, 1-23.
[http://dx.doi.org/10.1016/j.chroma.2015.12.076] [PMID: 26803907]
[36]
Al-Rimawi, F. A HPLC-UV method for determination of three pesticides in water. IJAC, 2016, 2(1), 9-16.
[37]
Tsochatzis, E.D.; Tzimou-Tsitouridou, R.; Menkissoglu-Spiroudi, U.; Dimitrios, G.K.; Papageorgiou, M. Development and validation of an HPLC-DAD method for the simultaneous determination of most common rice pesticides in paddy water systems. Int. J. Environ. Anal. Chem., 2012, 92(5), 548-560.
[http://dx.doi.org/10.1080/03067310903229943]
[38]
Harshit, D.; Charmy, K.; Nrupesh, P. Organophosphorus pesticides determination by novel HPLC and spectrophotometric method. Food Chem., 2017, 230, 448-453.
[http://dx.doi.org/10.1016/j.foodchem.2017.03.083] [PMID: 28407934]

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