Abstract
It is now widely believed that tumors originate from normal cells as a result of accumulated genetic/epigenetic changes. These alterations affect the signaling pathways at transcriptional and post-transcriptional level that drive cells into uncontrolled cell division, growth, and migration. Recent advancement of molecular technologies have yielded comprehensive gene expression profiling techniques that have successfully provided candidate diagnostic and prognostic markers in human cancers. Serial Analysis of Gene Expression (SAGE) is a technology to facilitate the measurement of mRNA transcripts of normal and malignant tissues in a non-biased and highly accurate and quantitative manner. SAGE produces a comprehensive gene expression portrait without a priori gene sequence information, leading to the identification of novel transcripts potentially involved in the pathogenesis of human cancer. In this review, we provide a brief outline of SAGE to underscore the advantages of the method relative to the other gene expression profiling approaches in cancer research. We also summarize the progression of recent gene expression profiling studies and discuss the current topics of SAGE analysis in cancer research for the development of novel therapeutic interventions.
Keywords: SAGE, gene expression profiling, transcriptome, pathway analysis, biomarker, prognosis, metastasis, chemo sensitivity
Current Pharmaceutical Biotechnology
Title: Application of Serial Analysis of Gene Expression in Cancer Research
Volume: 9 Issue: 5
Author(s): T. Yamashita, M. Honda and S. Kaneko
Affiliation:
Keywords: SAGE, gene expression profiling, transcriptome, pathway analysis, biomarker, prognosis, metastasis, chemo sensitivity
Abstract: It is now widely believed that tumors originate from normal cells as a result of accumulated genetic/epigenetic changes. These alterations affect the signaling pathways at transcriptional and post-transcriptional level that drive cells into uncontrolled cell division, growth, and migration. Recent advancement of molecular technologies have yielded comprehensive gene expression profiling techniques that have successfully provided candidate diagnostic and prognostic markers in human cancers. Serial Analysis of Gene Expression (SAGE) is a technology to facilitate the measurement of mRNA transcripts of normal and malignant tissues in a non-biased and highly accurate and quantitative manner. SAGE produces a comprehensive gene expression portrait without a priori gene sequence information, leading to the identification of novel transcripts potentially involved in the pathogenesis of human cancer. In this review, we provide a brief outline of SAGE to underscore the advantages of the method relative to the other gene expression profiling approaches in cancer research. We also summarize the progression of recent gene expression profiling studies and discuss the current topics of SAGE analysis in cancer research for the development of novel therapeutic interventions.
Export Options
About this article
Cite this article as:
Yamashita T., Honda M. and Kaneko S., Application of Serial Analysis of Gene Expression in Cancer Research, Current Pharmaceutical Biotechnology 2008; 9 (5) . https://dx.doi.org/10.2174/138920108785915102
DOI https://dx.doi.org/10.2174/138920108785915102 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Molecular Pathology of Sarcomas
Reviews on Recent Clinical Trials Amino Acid Degrading Enzymes and their Application in Cancer Therapy
Current Medicinal Chemistry Design, Synthesis and In-Vitro Cytotoxicity of Novel Platinum (II) Complexes with Phthalate as the Leaving Group
Letters in Drug Design & Discovery Tip60: Main Functions and Its Inhibitors
Mini-Reviews in Medicinal Chemistry Mitochondria-Mediated Oxidative Stress: Old Target for New Drugs
Current Medicinal Chemistry Non-invasive Biodiversified Sensors: A Modernized Screening Technology for Cancer
Current Pharmaceutical Design Recent Patents on Cell Cycle Regulatory Proteins
Recent Patents on Biotechnology Targeting the Most Upstream Site of Wnt Signaling Pathway Provides a Strategic Advantage for Therapy in Colorectal Cancer
Current Drug Targets Role of Pemetrexed and Platinums Combination in Patients with Non- Small Cell Lung Cancer
Current Drug Targets The Phosphoinositide 3-Kinase Pathway in Human Cancer: Genetic Alterations and Therapeutic Implications
Current Genomics Histone Deacetylases: Anti-Angiogenic Targets in Cancer Therapy
Current Cancer Drug Targets Preferentially Expressed Antigen in Melanoma (PRAME) and the PRAME Family of Leucine-Rich Repeat Proteins
Current Cancer Drug Targets Epidemiology and Pathology of Malignant Mesothelioma
Current Respiratory Medicine Reviews Cytokine Network: New Targeted Therapy for Pancreatic Cancer
Current Pharmaceutical Design C75, a Fatty Acid Synthase (FAS) Inhibitor
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Therapeutic Strategies for Targeting BRAF in Human Cancer
Reviews on Recent Clinical Trials Expression and Functions of Vasoactive Substances Regulated by Hypoxia-Inducible Factor-1 in Chronic Hypoxemia
Cardiovascular & Hematological Agents in Medicinal Chemistry Nanocarriers Based Anticancer Drugs: Current Scenario and Future Perceptions
Current Drug Targets Valproic Acid in the Complex Therapy of Malignant Tumors
Current Drug Targets Ribonucleases and ImmunoRNases as Anticancer Drugs
Current Pharmaceutical Design