Effects of the rad52 gene on recombination in Saccharomyces cerevisiae. [Comparison of. gamma. -, uv-induced meiotic and spontaneous mitotic recombination]
Effects of the rad52 mutation in Saccharomyces cerevisiae on meiotic, ..gamma..-ray-induced, uv-induced, and spontaneous mitotic recombination were studied. The rad52/rad52 diploids undergo premeiotic DNA synthesis; sporulation occurs but inviable spores are produced. Intra- and intergenic recombination during meiosis were examined in cells transferred from sporulation medium to vegetative medium at different time intervals. No intragenic recombination was observed at the hisl-1/hisl-315 and trp5-2/trp5-48 heteroalleles. Gene-centromere recombination was also not observed in rad52/rad52 diploids. No ..gamma..-ray-induced intragenic mitotic recombination is seen in rad52/rad52 diploids and uv-induced intragenic recombination is greatly reduced. However, spontaneous mitotic recombination is not similarly affected. The RAD52 gene thus functions in recombination in meiosis and in ..gamma..-ray and uv-induced mitotic recombination but not in spontaneous mitotic recombination.
- Research Organization:
- Rochester Univ., NY (USA). Dept. of Biology; Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
- DOE Contract Number:
- EY-76-C-02-3490
- OSTI ID:
- 6162720
- Report Number(s):
- UR-3490-1571; TRN: 79-012532
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis
Spontaneous mitotic recombination in the mms8-1 mutant of Saccharomyces cerevisiae
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
GENE MUTATIONS
RADIOSENSITIVITY EFFECTS
GENE RECOMBINATION
RADIOINDUCTION
MEIOSIS
GENETIC RADIATION EFFECTS
MITOSIS
SACCHAROMYCES CEREVISIAE
COMPARATIVE EVALUATIONS
EXPERIMENTAL DATA
GAMMA RADIATION
GENOTYPE
GRAPHS
IRRADIATION
ISOLATED VALUES
SPONTANEOUS MUTATIONS
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL DIVISION
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
FUNGI
GENETIC EFFECTS
INFORMATION
IONIZING RADIATIONS
MICROORGANISMS
MUTATIONS
NUMERICAL DATA
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550400 - Genetics
550700 - Microbiology