Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Journal of Andrology, Vol. 26, No. 6, November/December 2005
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.05045

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wakle, M. S.
Right arrow Articles by Khole, V. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wakle, M. S.
Right arrow Articles by Khole, V. V.

Monoclonal Antibody From Vasectomized Mouse Identifies a Conserved Testis-Specific Antigen TSA70

MONALI S. WAKLE*, SAURABH A. JOSHI{dagger} AND VRINDA V. KHOLE*

From the * Department of Gamete Immuno Biology, National Institute for Research in Reproductive Health, Mumbai, India; and the {dagger} Laboratory of Cellular and Developmental Biology, NIDDK, National Institute of Health, Bethesda, Maryland.

Correspondence to: Dr Vrinda V. Khole, Department of Gamete Immuno Biology, National Institute for Research in Reproductive Health, J M Street, Parel, Mumbai-400012, India (e-mail: vrindakhole{at}hotmail.com).


Vasectomy results in the occlusion of testicular outflow, leading to autoimmunity characterized by the production of antisperm antibodies (ASA). Reports on the rise in ASA following vasectomy in several species are available; however, not much is known about the specific sperm autoantigens to which postvasectomy antibodies are directed. In the present study, monoclonal antibodies were generated using a vasectomized mouse. One of the monoclonal antibodies, D5E5, identified an approximately 70-kd antigen localized on the principal piece of the tail and also on the tip of the acrosome of mouse sperm. The cognate antigen was expressed postmeiotically in a stage-specific manner during spermiogenesis, starting from step 8 of elongating spermatids during spermiogenesis up to mature spermatozoa. The protein was conserved across the species, as observed by its presence in rat, bull, marmoset, and human sperm. Following capacitation, the antigen on the head was seen to shift to the acrosomal region and was lost after the acrosome reaction. However, the localization on tip of the acrosome still persisted, which indicates that the antigen may play a role post-acrosome reaction in sperm egg interaction. Resistance to Triton X-100 solubilization indicates that TSA70 could be an acrosomal matrix protein. In addition, we observed a significant reduction in forward progressive motility of mouse sperm treated in vitro with D5E5. In view of its testis specificity, acrosome and tail localization, and conserved nature, TSA70 is likely to play an important role in sperm function.

     Key words: Vasectomy, testicular auto antigen, acrosomal matrix, capacitation




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
E. Prabagaran, A.H. Bandivdekar, V. Dighe, and V.P. Raghavan
HOXBES2: A Novel Epididymal HOXB2 Homeoprotein and Its Domain-Specific Association with Spermatozoa
Biol Reprod, February 1, 2007; 76(2): 314 - 326.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by The American Society of Andrology.