Genetic Variants of Interleukin-18 Promoter Gene in Coronary Artery Disease

Mageed, Lamiaa; Borai, Ibrahim H.; Hassan, Nahla S.; Shaker, Olfat G.; Ashour, Esmat; El Badrawy, Mohammed; Fawzi, Olfat M.

doi:10.21608/ejchem.2017.1337.1085

Genetic Variants of Interleukin-18 Promoter Gene in Coronary Artery Disease

Authors

¹ Biochemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Giza-Egypt

² Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

³ Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University

⁴ The National Heart Institute, Giza, Egypt

10.21608/ejchem.2017.1337.1085

Abstract

Background & objectives: Heart disease is impacted by ecological and inherited factors. Interleukin-18 (IL-18) is a pro-inflammatory cytokine that stimulates the immune reaction and induces the formation of atherosclerotic plaques. The aim of this study was to determine the relation between IL-18 promoter variants at (607C/A) and (137G/C) sites and coronary artery disease (CAD).
Subjects & Methods: A sum of one hundred and twenty Egyptian patients (Sixty with CAD and sixty without CAD) and fifty healthy controls were consolidated into the study. Genotyping of IL18 promoter gene was investigated by PCR - Specific Sequence primer (PCR-SSP) technique .
Results: The outcomes demonstrated that a significant association between lipid profiles and risk for CAD. At position-137, the recurrence of GG genotype was fundamentally connected with CAD. No qualifications in the genotypic and allelic frequencies amongst cases and controls were found for IL-18 (607C/A) promoter gene.
Conclusion: A relationship between IL-18 (137G/C) promoter gene and vulnerability to CAD was proposed and it might serve as susceptibility biomarkers in the pathogenesis of atherosclerosis.

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