Med. Weter. 75 (10), 599-604, 2019
full text
ANTONINA SOPIŃSKA, DAGNA SZUBSTARSKA |
Immunohistochemical identification of the bacterial antigen of Aeromonas hydrophila
in the kidneys and spleen of carps and evaluation of the activity of kidney phagocytes
after intraperitoneal and immersion immunization |
The aim of this study was to evaluate the activity of kidney phagocytes after immunization of fish with
inactivated bacteria of Aeromonas hydrophila and to determine the location and permanence of the bacterial
antigen in the lymphoid organs, the kidney and the spleen, of carps immunized intraperitoneally (group I) and
by immersion (group II) at 12oC. We examined the absorption of bacteria by kidney leucocytes (PK%), the
killing capacity of phagocytes (IB) and chemiluminescent activity (CL). Evaluations were performed 12 h, as
well as 3, 7, 14 and 28 days after immunization. At all these time points, the values of the parameters examined
were higher in fish immunized intraperitoneally than in those immunized by immersion. The absorption of
bacterial cells by kidney phagocytes was particularly high after 14 days after immunization in fish group I
whereas their killing and chemiluminescent activity was particularly high in 7 days after immunization in
group I. Immunohistochemical examination revealed an extensive distribution and large amounts of the antigen
in the interstitial part and melanomacrophage centers of the kidneys and spleen after the intraperitoneal
administration of the antigen, By contrast, small amounts of the antigen were found in these organs after it
had been administered by immersion. |
Keywords: carp, immunization, phagocytosis, Aeromonas hydrophila, antigen location |