ANTI-CANCER POTENTIAL OF COPPER OXIDE NANOPARTICLES AGAINST MURINE MAMMARY ADENOCARCINOMA (AMN-3) CELLS

This study was designed to evaluate the cytotoxic effect of copper nanoparticles in murine adenocarcinoma cells (AMN-3). The exposure period of cell line was performed at 24 hr in a microtitration plate under complete sterile conditions. Different concentrations were used started from 0.39 μgmL^-1to 50 μgmL^-1 in three independent experiments. First, the cells were stained by MTT and the absorbance were measured using Elisa reader at 492 nm. The treatment with nanoparticles showed a significant inhibition (P< 0.05) on cells and the effect was concentration dependent, the highest inhibition was 88% at 50 μgmL^-1, while was 15% at 0.39 μgmL^-1 and the inhibitory concentration 50 was 1.5 μgmL^-1. The cell death was evaluated in cell line after a treatment with CUNPs through two types of assessments, which were mitochondrial membrane potential assay and acridine orange- ethidium bromide dual staining assays. Results revealed that the tested substances showed a potent inhibitory cytotoxic effect against the proliferation of AMN-3 cells through apoptosis.