Microbial effects in promoting the smectite to illite reaction: Role of organic matter intercalated in the interlayer

Abstract

Cysteine and toluene as model organic molecules were intercalated into Fe-rich smectite (nontronite, NAu-2). The illitization of these intercalated smectites as induced by microbial reduction of structural Fe³⁺ was investigated. Iron-reducing bacterium Shewanella putrefaciens CN32 was incubated with lactate as the sole electron donor and structural Fe³⁺ in cysteine- and toluene-intercalated NAu-2 (referred to as cysteine-NAu-2 and toluene-NAu-2 hereafter) as the sole electron acceptor. Anthraquinone- 2, 6-disulfonate (AQDS) was used as an electron shuttle in bicarbonate buffer. The extent of Fe³⁺ reduction in cysteine-NAu-2 and toluene-NAu-2 was 15.7 and 5.4%, respectively, compared to 20.5% in NAu-2 without organic matter intercalation. In the bioreduced NAu-2, X-ray diffraction, and scanning and transmission electron microscopy did not detect any discrete illite, although illite/ smectite mixed layer or high charge smectite phases were observed. In bioreduced cysteine-NAu-2, discrete illite and siderite formed. In contrast, bioreduction of toluene-NAu-2 did not result in any mineralogical changes. The contrasting bioreduction results between cysteine- and toluene-intercalated nontronite may be ascribed to the nature of organic matter-bacteria interactions. Whereas cysteine is an essential amino acid for bacteria and can also serve as an electron shuttle, thus enhancing the extent of Fe³⁺ bioreduction and illitization, toluene is toxic and inhibits Fe³⁺-reducing activity. This study, therefore, highlights the significant role of organic matter in promoting the smectite to illite reaction under conditions typical of natural environments (i.e., non-growth condition for bacteria).