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Organic Phosphates Analyzer Using High-Performance Anion-Exchange Chromatography and a Postcolumn Phosphomolybdic Acid Reaction

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Abstract

An organic phosphates analyzer was developed based on high-performance anion-exchange chromatography and a postcolumn phosphomolybdic acid reaction. The organic phosphates were separated on an Asahipak NH2P-50 column and then introduced into a postcolumn reaction detector in which organic phosphates were heated at 170°C in 2.0 M sulfuric acid for 5.8 min. The resultant orthophosphate was monitored at 830 nm after a selective reaction with a molybdenum reagent. When l.Onmol of each sugar phosphate solution was employed (injection volumn, 10 μl) the relative standard deviations were 1.9 - 2.6% (n=8). The detection limits (S/ N=3) of the organic phosphates were ca. 100 pmol as the injected amount.

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Author notes

  1. Terumi Nakajima

    Present address: Suntory Institute for Bioorganic Research, 1-1-1, Wakayamadai, Shimamoto, Mishima, Osaka, 618, Japan

Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo, 113, Japan

    Yoshihiko Ikeguchi, Hiroshi Nakamura & Terumi Nakajima

Authors
  1. Yoshihiko Ikeguchi
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  2. Hiroshi Nakamura
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  3. Terumi Nakajima
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Ikeguchi, Y., Nakamura, H. & Nakajima, T. Organic Phosphates Analyzer Using High-Performance Anion-Exchange Chromatography and a Postcolumn Phosphomolybdic Acid Reaction. ANAL. SCI. 9, 653–655 (1993). https://doi.org/10.2116/analsci.9.653

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  • DOI: https://doi.org/10.2116/analsci.9.653

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