Skip to main content
SpringerLink
Log in

In situ Observation of Desorption Reaction of Cytochrome c from Solid/Liquid Interfaces with Slab Optical Waveguide Spectroscopy

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

An automated solution exchange (SE) mechanism has been introduced to slab optical waveguide spectroscopy to estimate the protein immobilizing ability of a slab optical waveguide (SOWG) surface. In each SE process, SOWG spectral change in absorbance at peak position of cytochrome c (cyt.c) Soret band at 409 nm was observed to analyze the desorption ratio of cyt.c adsorbed on SOWG surface. Continuous SE processes for 100 times have successfully brought us a kind of master desorption curve of cyt.c, which was well fitted by a double exponential equation, indicating the existence of three kinds of adsorbed states, including weakly adsorbed, strongly adsorbed, and immobilized cyt.c. The present results showed that around 30 times SE processes were enough to anticipate the ratio of desorbed and immobilized amounts of cyt.c adsorbed on SOWG surface.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. Yeh and T. Kuwana, Chem. Lett., 1977, 6, 1145.

    Article  Google Scholar 

  2. E. F. Bowden, F. M. Hawkridge, and H. N. Blount, J. Electroanal. Chem., 1984, 161, 355.

    Article  CAS  Google Scholar 

  3. A. El Kasmi, M. C. Leopold, R. Galligan, R. T. Robertson, S. S. Saavedra, K. Kl Kacemi, and E. F. Bowden, Electrochem. Commun., 2002, 4, 177.

    Article  CAS  Google Scholar 

  4. N. Matsuda, J. H. Santos, A. Takatsu, and K. Kato, Thin Solid Films, 2003, 438-439, 403.

    Article  Google Scholar 

  5. Y. Ayato, A. Takatsu, K. Kato, and N. Matsuda, Jpn. J. Appl. Phys., 2008, 47, 1333.

    Article  CAS  Google Scholar 

  6. Z.-M. Qi, N. Matsuda, T. Yoshida, H. Asano, A. Takatsu, and K. Kato, Opt. Lett., 2002, 27, 2001.

    Article  PubMed  Google Scholar 

  7. J. H. Santos, N. Matsuda, Z.-M. Qi, T. Yoshida, A. Takatsu, and K. Kato, Anal. Sci., 2013, 19, 199.

    Article  Google Scholar 

  8. N. Matsuda and H. Okabe, IEICE Trans. Electron., 2013, E96-C, 389.

    Article  Google Scholar 

  9. N. Matsuda and H. Okabe, IEICE Trans. Electron., 2015, E98-C, 152.

    Article  Google Scholar 

  10. S. Ong, X. Zhao, and K. B. Eisenthal, Chem. Phys. Lett., 1992, 191, 327.

    Article  CAS  Google Scholar 

  11. K. Leung, I. M. B. Nielsen, and L. J. Criscenti, J. Am. Chem. Soc., 2009, 131, 18358.

    Article  CAS  PubMed  Google Scholar 

  12. X. Liu, J. Cheng, X. Lua, and R. Wanga, Phys. Chem. Chem. Phys., 2014, 16, 26909

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge Ms. Keiko Shimizu for her assistance in data analysis.

Author information

Authors and Affiliations

  1. Faculty of Humanity-Oriented Science and Engineering, Kindai University, 11-6, Kayanomori, Iizuka, Fukuoka, 820-8555, Japan

    Shinya Taguchi, Hirofumi Kawazumi & Naoki Matsuda

  2. Materials Chemistry Course, Department of Creative Engineering, National Institute of Technology, Kitakyushu College, 5-20-1, Shii, Kokuraminami, Kitakyushu, Fukuoka, 802-0985, Japan

    Toshihiko Nagamura

  3. Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology, 807-1 Shukumachi, Tosu, Saga, 841-0052, Japan

    Hirotaka Okabe

Authors
  1. Shinya Taguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hirofumi Kawazumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshihiko Nagamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hirotaka Okabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Naoki Matsuda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Toshihiko Nagamura or Naoki Matsuda.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Taguchi, S., Kawazumi, H., Nagamura, T. et al. In situ Observation of Desorption Reaction of Cytochrome c from Solid/Liquid Interfaces with Slab Optical Waveguide Spectroscopy. ANAL. SCI. 33, 461–463 (2017). https://doi.org/10.2116/analsci.33.461

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.33.461

Keywords

Search

Navigation