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Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds

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Abstract

Immobilization of functional peptides on polymer material is necessary to produce cell-selective scaffolds. However, the expected effects of peptide immobilization differ considerably according to the properties of selected polymers. To understand such combinational effects of peptides and polymers, varieties of scaffolds including a combination of six types of poly(ɛ-caprolactone-co-D,L-lactide) and four types of cell-selective adhesion peptides were fabricated and compared. On each scaffold, the scaffold properties (i.e.. mechanical) and their biological functions (i.e.. fibroblast-/ endothelial cell-/smooth muscle cell-selective adhesion) were measured and compared. The results showed that the cell adhesion performances of the peptides were considerably enhanced or inhibited by the combination of peptide and polymer properties. In the present study, we illustrated the combinational property effects of peptides and polymers using multi-parametric analyses. We provided an example of determining the best scaffold performance for tissue-engineered medical devices based on quantitative data-driven analyses.

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References

  1. G. P. Chen, T. Ushida, and T. Tateishi, J. Biomed. Mater. Res., 2000, 51, 273.

    Article  CAS  PubMed  Google Scholar 

  2. T. Yokota, H. Ichikawa, G. Matsumiya, T. Kuratani, T. Sakaguchi, S. Iwai, Y. Shirakawa, K. Torikai, A. Saito, E. Uchimura, N. Kawaguchi, N. Matsuura, and Y. Sawa, J. Thorac. Cardiovasc. Surg., 2008, 136, 900.

    Article  PubMed  Google Scholar 

  3. K. O. Cardinal and S. K. Williams, Tissue Eng. Part A, 2009, 15, 3869.

    Article  PubMed  Google Scholar 

  4. J. Aoki, P. W. Serruys, H. van Beusekom, A. T. L. Ong, E. P. McFadden, G. Sianos, W. J. van der Giessen, E. Regar, P. J. de Feyter, H. R. Davis, S. Rowland, and M. J. B. Kutryk, J. Am. Coll. Cardiol., 2005, 45, 1574.

    Article  CAS  PubMed  Google Scholar 

  5. M. D. Pierschbacher and E. Ruoslahti, Nature, 1984, 309, 30.

    Article  CAS  PubMed  Google Scholar 

  6. J. Graf, R. C. Ogle, F. A. Robey, M. Sasaki, G. R. Martin, Y. Yamada, and H. K. Kleinman, Biochemistry, 1987, 26, 6896.

    Article  CAS  PubMed  Google Scholar 

  7. M. Yin, Y. Yuan, C. Liu, and J. Wang, Biomaterials, 2009, 30, 2764.

    Article  CAS  PubMed  Google Scholar 

  8. D. Kong, L. G. Melo, A. A. Mangi, L. Zhang, M. Lopez-Ilasaca, M. A. Perrella, C. C. Liew, R. E. Pratt, and V. J. Dzau, Circulation, 2004, 109, 1769.

    Article  CAS  PubMed  Google Scholar 

  9. K. Kanie, Y. Narita, Y. Z. Zhao, F. Kuwabara, M. Satake, S. Honda, H. Kaneko, T. Yoshioka, M. Okochi, H. Honda, and R. Kato, Biotechnol. Bioeng., 2012, 109, 1808.

    Article  CAS  PubMed  Google Scholar 

  10. M. Khan, J. Yang, C. Shi, J. Lv, Y. Feng, and W. Zhang, Acta Biomater., 2015, 20, 69.

    Article  CAS  PubMed  Google Scholar 

  11. F. Kuwabara, Y. Narita, A. Yamawaki-Ogata, K. Kanie, R. Kato, M. Satake, H. Kaneko, H. Oshima, A. Usui, and Y. Ueda, Ann. Thorac. Surg., 2012, 93, 156.

    Article  PubMed  Google Scholar 

  12. K. Kanie, R. Kato, Y. Z. Zhao, Y. Narita, M. Okochi, and H. Honda, J. Pept. Sci., 2011, 17, 479.

    Article  CAS  PubMed  Google Scholar 

  13. C. S. Chen, M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber, Science, 1997, 276, 1425.

    Article  CAS  PubMed  Google Scholar 

  14. D. E. Ingber, Prog. Biophys. Mol Biol., 2008, 97, 163.

    Article  PubMed  PubMed Central  Google Scholar 

  15. A. J. Engler, S. Sen, H. L. Sweeney, and D. E. Discher, Cell, 2006, 126, 677.

    Article  CAS  PubMed  Google Scholar 

  16. K. Uto, S. S. Mano, T. Aoyagi, and M. Ebara, ACS Biomater. Sci. Eng., 2016, 2, 446.

    Article  CAS  PubMed  Google Scholar 

  17. S. S. Mano, K. Uto, T. Aoyagi, and M. Ebara, AIMS Mater. Sci., 2016, 3, 66.

    Article  CAS  Google Scholar 

  18. R. Kurimoto, K. Kanie, N. Idota, M. Hara, S. Nagano, T. Tsukahara, Y. Narita, H. Honda, M. Naito, M. Ebara, and R. Kato, Int. J. Polym. Sci., 2016, 2016, 9.

    Article  Google Scholar 

  19. K. Uto, T. Muroya, M. Okamoto, H. Tanaka, T. Murase, M. Ebara, and T. Aoyagi, Sci. Technol. Adv. Mater., 2012, 13.

    Google Scholar 

  20. A. R. Cameron, J. E. Frith, and J. J. Cooper-White, Biomaterials, 2011, 32, 5979.

    Article  CAS  PubMed  Google Scholar 

  21. B. Li, J. X. Chen, and J. H. C. Wang, J. Biomed. Mater. Res. A, 2006, 79A, 989.

    Article  CAS  Google Scholar 

  22. H. Sasaki, I. Takeuchi, M. Okada, R. Sawada, K. Kanie, Y. Kiyota, H. Honda, and R. Kato, Plos One, 2014, 9.

    Google Scholar 

  23. K. Ito, A. Hikida, S. Kawai, L. Vu Thi Tuyet, T. Motoyama, S. Kitagawa, Y. Yoshikawa, R. Kato, and Y. Kawarasaki, Nat. Commun., 2013, 4.

    Google Scholar 

  24. J. Friedman, T. Hastie, and R. Tibshirani, J. Stat. Softw., 2010, 33, 1.

    Article  PubMed  PubMed Central  Google Scholar 

  25. S. Kawashima, P. Pokarowski, M. Pokarowska, A. Kolinski, T. Katayama, and M. Kanehisa, Nucleic Acids Res., 2008, 36, D202.

    Article  CAS  PubMed  Google Scholar 

  26. K. Ye, X. Wang, L. P. Cao, S. Y. Li, Z. H. Li, L. Yu, and J. D. Ding, Nano Lett., 2015, 15, 4720.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by JSPS KAKENHI. Grant Number JP 15K21070. Part of this work was supported by the Nanotechnology Platform Program (Molecule and Polymer Synthesis) of the Ministry of Education. Culture. Sports. Science and Technology (MEXT). Japan. R. K. acknowledges the technical support of Y. Kondo. M. Okada, and A. Ooguchi (Nagoya University). We would like to thank Editage (www. editage.jp) for English language editing.

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Authors and Affiliations

  1. Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan

    Rio Kurimoto, Masanobu Naito & Mitsuhiro Ebara

  2. International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan

    Rio Kurimoto, Koichiro Uto & Mitsuhiro Ebara

  3. Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi, 464-8601, Japan

    Kei Kanie, Shun Kawai & Ryuji Kato

  4. Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University’, Furo-cho, Chikusa, Nagoya, Aichi, 464-8603, Japan

    Mitsuo Hara

  5. Nagoya University Venture Business Laboratory, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi, 464-8603, Japan

    Shiisaku Nagano

  6. Department of Cardiac Surgery’, Nagoya University Graduate School of Medicine, 65 Turumai-cho, Showa, Nagoya, Aichi, 466-8550, Japan

    Yuji Narita

  7. Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi, 464-8603, Japan

    Hiroyuki Honda

  8. Structural Materials Unit, Research Center for Strategic Materials, NIMS, 1-2-1 Sengen, Tsukuba, Ibaraki, 305-0047, Japan

    Masanobu Naito

  9. Graduate School of Industrial Science and Technology', Tokyo University of Science, Tokyo, 125-8585, Japan

    Mitsuhiro Ebara

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  1. Rio Kurimoto
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  2. Kei Kanie
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  3. Koichiro Uto
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  4. Shun Kawai
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  5. Mitsuo Hara
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  6. Shiisaku Nagano
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  7. Yuji Narita
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  8. Hiroyuki Honda
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  9. Masanobu Naito
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  10. Mitsuhiro Ebara
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  11. Ryuji Kato
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Correspondence to Ryuji Kato.

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Kurimoto, R., Kanie, K., Uto, K. et al. Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds. ANAL. SCI. 32, 1195–1202 (2016). https://doi.org/10.2116/analsci.32.1195

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  • DOI: https://doi.org/10.2116/analsci.32.1195

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