Abstract
In this paper, an egg-shell membrane has been used for efficient immobilization and stabilization of glucose oxidase. This membrane was used for developing a simple and reusable method for estimation of glucose in biological samples. The proposed sensor was effectively used in a wide glucose concentration range (1–1000 mM) with fast response time of 70 s for higher concentrations and 120 s for lower concentrations. The results of response study for the fabricated sensor show limit of detection of 4.761 mM with high sensitivity over the entire concentration range (1–1000 mM). Most interestingly, the membrane used in the fabricated sensor could be repeatedly used for glucose analysis 150 times and it exhibited a shelf-life of more than 6 weeks. The proposed sensor was also demonstrated for estimation of glucose in human blood samples.
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Acknowledgments
The authors are thankful to IIT Delhi, India for providing facilities, infrastructure and intramural financial support. This study was also supported by the Department of Biotechnology, Government of India (Grant No. BT/PR7190/PID/6/719/2012). The author Appan Roychoudhury is a recipient of Junior Research Fellowship from the Indian Institute of Technology Delhi.
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Singh, A.K., Roychoudhury, A. & Jha, S.K. Reusable Glucose Sensor Based on Enzyme Immobilized Egg-shell Membrane. ANAL. SCI. 32, 1077–1082 (2016). https://doi.org/10.2116/analsci.32.1077
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DOI: https://doi.org/10.2116/analsci.32.1077