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A Comparative Fluorescent Beacon-based Method for Serum microRNA Quantification

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Abstract

Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 μL healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number.

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Correspondence to Janakiraman Narayanan.

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Beta, M., Krishnakumar, S., Elchuri, S.V. et al. A Comparative Fluorescent Beacon-based Method for Serum microRNA Quantification. ANAL. SCI. 31, 231–235 (2015). https://doi.org/10.2116/analsci.31.231

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  • DOI: https://doi.org/10.2116/analsci.31.231

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