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Naked-eye Detection of Amyloid Aggregates Using Gold Nanoparticles Modified with Amyloid Beta Antibody

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Abstract

We developed a rapid method for estimating the amyloid beta (Aβ)-conformation state related with Alzheimer’s disease. We prepared gold nanoparticle (AuNP)-Aβ antibody conjugates treated with bovine serum albumin to stabilize their dispersibility in a buffer. The prepared AuNPs were precipitated in the presence of Aβ aggregates, such as oligomers and fibrils. Aβ monomers did not precipitate AuNPs. The formation of AuNP precipitates by Aβ aggregates could be confirmed by the naked eye within 1 h.

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Authors and Affiliations

  1. PRESTO, Japan Science and Technology Agency, 3-5 Sanbancho, 102-0075, Chiyoda, Tokyo, Japan

    Masafumi Sakono

  2. Bioengineering Laboratory, RIKEN Institute, 2-1 Hirosawa, 351-0198, Wako, Saitama, Japan

    Masafumi Sakono, Tamotsu Zako & Mizuo Maeda

Authors
  1. Masafumi Sakono
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  2. Tamotsu Zako
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  3. Mizuo Maeda
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Correspondence to Masafumi Sakono.

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Sakono, M., Zako, T. & Maeda, M. Naked-eye Detection of Amyloid Aggregates Using Gold Nanoparticles Modified with Amyloid Beta Antibody. ANAL. SCI. 28, 73–76 (2012). https://doi.org/10.2116/analsci.28.73

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  • DOI: https://doi.org/10.2116/analsci.28.73

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