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Microfluidic Devices for Electrochemical Measurement of Photosynthetic Activity of Cyanobacteria Microcystis Cells

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Abstract

A microfluidic device with analytical chambers for electrochemical measurements has been employed to detect photosynthetic activity at single cell level. The flowing cells (Microcystis viridis) in a main channel are individually guided to the chamber with microelectrodes by an electrophoretic manipulation. The reduction current of oxygen was continuously monitored to determine the photosynthetic activity upon light irradiation. The average rates for oxygen generation were estimated and found to be 10−18 mol/s level.

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Author notes

  1. Tomoaki Itayama

    Present address: Graduate School of Engineering, Nagasaki University, 1-14 Bunnkyo, 852-8521, Nagasaki, Japan

Authors and Affiliations

  1. National Institute for Environmental Studies, 16-2 Onogawa, 305-8506, Tsukuba, Ibaraki, Japan

    Masahiro Koide & Tomoaki Itayama

  2. Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki, 980-8579, Aoba, Sendai, Japan

    Masahiro Koide, Yoshiko Horiguchi, Kuniaki Nagamine, Hitoshi Shiku & Tomokazu Matsue

  3. Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, 678-1297, Kamigori, Ako, Hyogo, Japan

    Tomoyuki Yasukawa

Authors
  1. Masahiro Koide
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  2. Tomoyuki Yasukawa
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  3. Yoshiko Horiguchi
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  4. Kuniaki Nagamine
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  5. Hitoshi Shiku
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  6. Tomokazu Matsue
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  7. Tomoaki Itayama
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Corresponding authors

Correspondence to Masahiro Koide or Tomoaki Itayama.

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Koide, M., Yasukawa, T., Horiguchi, Y. et al. Microfluidic Devices for Electrochemical Measurement of Photosynthetic Activity of Cyanobacteria Microcystis Cells. ANAL. SCI. 28, 69–72 (2012). https://doi.org/10.2116/analsci.28.69

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  • DOI: https://doi.org/10.2116/analsci.28.69

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