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A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes

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Abstract

Piezoelectric inkjet printing of polymers and proteins holds great promise for fabrication of miniaturized bioelectronic devices, such as biochips and biosensors. In this study, a bienzymatic glucose biosensor prototype based on poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) (PEDOT-PSS), glucose oxidase (GOD), and horseradish peroxidase (HRP) was fabricated by a piezoelectric inkjet printer. An aqueous bioelectrical ink containing PEDOT-PSS, GOD, and HRP was prepared and printed on an indium-tin-oxide (ITO)-coated poly(ethylene terephthalate) (PET) film. The PEDOT-PSS/GOD/HRP sensor was covered with a cellulose acetate membrane. The use of bienzymatic sensing combined with conducting polymers via piezoelectric inkjet printing showed a synergistic effect resulting in significant amplification of the response signal. The glucose sensor reached steady-state current density within 3 s, indicating a fast response time, and exhibited a linear dose-dependent electrochemical response with high sensitivity. The overall result demonstrates that a glucose sensor with high sensitivity could be readily fabricated by a piezoelectric inkjet printing system.

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Authors and Affiliations

  1. Department of Chemical Engineering and Department of Bionanotechnology, Hanyang University, 426-791, Ansan, Gyeonggi-do, Republic of Korea

    Yeon Hee Yun, Byung Kook Lee, Ji Suk Choi & Yong Woo Cho

  2. Departments of Pharmaceutics and Biomedical Engineering, Purdue University, 47907, West Lafayette, IN, USA

    Sungwon Kim & Kinam Park

  3. Department of Materials Engineering, Hanyang University, 426-791, Ansan, Gyeonggi-do, Republic of Korea

    Bongyoung Yoo

  4. Department of Applied Chemistry, Hanyang University, 426-791, Ansan, Gyeonggi-do, Republic of Korea

    Yong Shin Kim

Authors
  1. Yeon Hee Yun
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  2. Byung Kook Lee
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  3. Ji Suk Choi
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  4. Sungwon Kim
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  5. Bongyoung Yoo
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  6. Yong Shin Kim
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  7. Kinam Park
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  8. Yong Woo Cho
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Correspondence to Yong Woo Cho.

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Yun, Y.H., Lee, B.K., Choi, J.S. et al. A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes. ANAL. SCI. 27, 375–379 (2011). https://doi.org/10.2116/analsci.27.375

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  • DOI: https://doi.org/10.2116/analsci.27.375

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