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Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

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Abstract

Capillary electrophoresis (CE) coupling with a tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) electrochemiluminescence (ECL) detection technique was developed for the analysis of two β-blockers, atenolol (AT) and metoprolol (ME). The parameters that influence the separation and detection, including the buffer pH and concentration, the separation voltage, the detection potential and Ru(bpy)32+ concentration, were optimized in detail. The calibration curve was linear over a concentration range of two or three orders of magnitude for the two β-blockers. The detection limits for AT and ME were 0.075 and 0.005 μM (S/N = 3). The relative standard deviations (n = 8) of the ECL intensity and the migration time were 2.65 and 0.22% for AT, 2.82 and 0.34% for ME, respectively. The proposed method was applied to determine AT and ME in spiked urine samples; satisfactory results were obtained.

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China

    Jianshe Huang, Jinying Sun, Xiaoguang Zhou & Tianyan You

Authors
  1. Jianshe Huang
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  2. Jinying Sun
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  3. Xiaoguang Zhou
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  4. Tianyan You
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Correspondence to Tianyan You.

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Huang, J., Sun, J., Zhou, X. et al. Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection. ANAL. SCI. 23, 183–188 (2007). https://doi.org/10.2116/analsci.23.183

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  • DOI: https://doi.org/10.2116/analsci.23.183

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