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Evaluation of an ODS Column Modified with Zwitterionic/ Nonionic Mixed Surfactants and Its Application to Direct Injection Determination of Inorganic Anions

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Abstract

An octadecylsilica (ODS) column modified with zwitterionic/nonionic mixed surfactants was evaluated for the direct injection determination of inorganic anions in biological fluids by ion chromatography. A zwitterionic surfactant (sulfobetaine-type) and a nonionic surfactant (polyoxyethylene-type) were used for a stationary-phase modification. When aqueous electrolyte solutions with concentrations of sub-mM to several mM were used as a mobile phase, the zwitterionic surfactant coated on the ODS surface exhibited unique separation selectivity for ionic species, while the nonionic surfactant coated on the ODS might have formed a hydrophilic network over the ODS surface and restricted matrix proteins from adsorbing on the stationary phase. Consequently, the mixed surfactant-modified column system allowed an efficient ion chromatographic separation of inorganic anions as well as a size-exclusive removal of column-fouling proteins. This separation system was applied to the direct injection determination of UV-absorbing anions in human saliva. The detection limits for nitrite, nitrate, iodide and thiocyanate were 3.1, 2.7, 4.5 and 25 μM, respectively, with UV detection at 210 nm (injection volume; 20 μl), and their relative standard deviations for 5 replicate measurements of saliva samples spiked with 100 μM each of those anions were 1.4, 0.9, 2.2 and 5.5%, respectively.

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Author notes

  1. Koichi Chiba

    Present address: National Institute of Advanced Industrial Science and Technology, Tsukuba Central 3, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8563, Japan

Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Takuya Hasegawa, Tomonari Umemura, Akira Koide, Koichi Chiba & Hiroki Haraguchi

  2. Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan

    Yuji Ueki & Kin-ichi Tsunoda

Authors
  1. Takuya Hasegawa
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  2. Tomonari Umemura
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  3. Akira Koide
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  4. Koichi Chiba
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  5. Yuji Ueki
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  6. Kin-ichi Tsunoda
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  7. Hiroki Haraguchi
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Correspondence to Tomonari Umemura.

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Hasegawa, T., Umemura, T., Koide, A. et al. Evaluation of an ODS Column Modified with Zwitterionic/ Nonionic Mixed Surfactants and Its Application to Direct Injection Determination of Inorganic Anions. ANAL. SCI. 21, 913–916 (2005). https://doi.org/10.2116/analsci.21.913

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  • DOI: https://doi.org/10.2116/analsci.21.913

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