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Rapid Formation of Arrayed Cells on an Electrode with Microwells by a Scanning Electrode Based on Positive Dielectrophoresis

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Abstract

We have developed a simple and rapid formation of a cell-based array on microwell array electrodes by an attractive force of positive dielectrophoresis (p-DEP), even after removing an upper disk electrode stick that was used as a counter electrode to the microwell array electrodes. The attractive force of p-DEP generated by the scanning of the disk electrode allows the formation of a cell-based array on all microwell arrays. We demonstrated an exploration of target cells spiked with a low ratio after removing the disk electrode.

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Acknowledgments

This work was partly supported by JSPS KAKENHI Grant Nos. 18K19006, 17H03468, and 18H05993.

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Authors and Affiliations

  1. Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo, 678-1297, Japan

    Hiroki Okayama, Masato Suzuki & Tomoyuki Yasukawa

  2. Division of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurima-Machiya-cho, Tsu, Mie, 514-8507, Japan

    Masahiro Tomita

Authors
  1. Hiroki Okayama
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  2. Masahiro Tomita
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  3. Masato Suzuki
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  4. Tomoyuki Yasukawa
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Correspondence to Tomoyuki Yasukawa.

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Okayama, H., Tomita, M., Suzuki, M. et al. Rapid Formation of Arrayed Cells on an Electrode with Microwells by a Scanning Electrode Based on Positive Dielectrophoresis. ANAL. SCI. 35, 701–704 (2019). https://doi.org/10.2116/analsci.19N002

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  • DOI: https://doi.org/10.2116/analsci.19N002

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