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Derivative Matrix Isopotential Synchronous Fluorescence Spectroscopy for the Direct Determination of 1-Hydroxypyrene as a Urinary Biomarker of Exposure to Polycyclic Aromatic Hydrocarbons

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Abstract

Urinary 1-hydroxypyrene is a biomarker in the measurement of human exposure to polycyclic aromatic hydrocarbons. A rapid and simple derivative isopotential synchronous fluorescence method was developed for the direct determination of 1-hydroxypyrene in urine. A length of iso-intensity route was scanned on the three-dimensional fluorescence spectrum of urine and this result was combined with that from derivative technique. Thus the strong background signals of urine were removed and the 1-hydroxypyrene can be determined directly in urine without tedious pre-separation. The derivative isopotential synchronous fluorescence spectrum was directly obtained from a single scan on a spectrofluorometer, which further simplified isopotential synchronous fluorescence technique. The recoveries of 93% to 115% were obtained for 1-hydroxypyrene added to urine.

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Authors and Affiliations

  1. The Key Laboratory of Analytical Sciences of MOE and Department of Chemistry, Xiamen University, Xiamen, 361005, China

    Yao-Qun Li, Wei Sui, Chun Wu & Li-Jun Yu

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  1. Yao-Qun Li
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  2. Wei Sui
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  3. Chun Wu
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  4. Li-Jun Yu
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Correspondence to Yao-Qun Li.

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Li, YQ., Sui, W., Wu, C. et al. Derivative Matrix Isopotential Synchronous Fluorescence Spectroscopy for the Direct Determination of 1-Hydroxypyrene as a Urinary Biomarker of Exposure to Polycyclic Aromatic Hydrocarbons. ANAL. SCI. 17, 167–170 (2001). https://doi.org/10.2116/analsci.17.167

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  • DOI: https://doi.org/10.2116/analsci.17.167

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