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Determination of Hydroxylated Polycyclic Aromatic Hydrocarbons in Airborne Particulates by High-Performance Liquid Chromatography with Fluorescence Detection

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Abstract

This paper describes a highly sensitive and selective method for the determination of hydroxylated polycyclic aromatic hydrocarbons (PAHs-OH) in airborne particulates. PAHs-OH were extracted from airborne particulates with ethanol by ultrasonication. The extractant was further cleaned up by solid phase extraction (SPE) with an aminopropylsilica cartridge, and subsequently analyzed by reversed-phase high-performance liquid chromatography with fluorescence detection. 2-Hydroxy-1-acetonaphthone was used as an internal standard. By the proposed method, 2-hydroxyfluorene and 1-hydroxypyrene were identified in airborne particulates and their concentrations were determined for the first time.

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Authors and Affiliations

  1. Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Naoya Kishikawa, Seigo Morita, Mitsuhiro Wada, Yoshihito Ohba, Kenichiro Nakashima & Naotaka Kuroda

Authors
  1. Naoya Kishikawa
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  2. Seigo Morita
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  3. Mitsuhiro Wada
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  4. Yoshihito Ohba
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  5. Kenichiro Nakashima
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  6. Naotaka Kuroda
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Correspondence to Naotaka Kuroda.

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Kishikawa, N., Morita, S., Wada, M. et al. Determination of Hydroxylated Polycyclic Aromatic Hydrocarbons in Airborne Particulates by High-Performance Liquid Chromatography with Fluorescence Detection. ANAL. SCI. 20, 129–132 (2004). https://doi.org/10.2116/analsci.20.129

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  • DOI: https://doi.org/10.2116/analsci.20.129

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