National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Earthworms have been found enormously with the presence of symbionts in various organs. The earthworm, Eudrilus eugeniae is harbouring symbionts in the prostate gland. The molecular analysis of 16SrRNA exposed the presence of two bacterial isolates in the prostate gland, one specific for the prostate gland and the other one common harbouring in the clitellum was found out. Gram negative, rod shaped, Catalase negative and Oxidase positive bacterium was identified to be specific for the prostate gland. 16S rRNA gene sequences of the prostate specific isolate indicated its relatedness to the genus Enterobacteriaeceae. Gene sequence assembled and phylogenetic analysis revealed the isolate with 99% similarity with Enterobacter sp. The isolate harboured in the clitellum and prostate indicated its relatedness to Bacillus cereus. Both the bacterial isolates were found to be present in the entire life stages of earthworm. Predominance of the bacterium was found in the stage of cocoon, with least presence at the juvenile stage. Experimental studies explored the importance of prostate specific isolate associated with the fecundity rate.
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